[Molecular-biologic analysis of avian influenza virus isolates which caused epizootics on the south of West Siberia and in Crimea].

The objective of the study was to determine reasons of poultry deaths in Crimea Republic in December 2005 as well as isolation, identification, and comparative analysis of pathogens, which caused epizootics in Siberia and Crimea. During epizootic in poultry in North-East Crimea highly pathogenic avian influenza virus H5N1 was isolated. Phylogenetic analysis of RNA sequences revealed that they belong to one big cluster.

[Characterization of the natural foci of Congo-Crimean hemorrhagic fever on the territory of Ukraine].

The natural foci of the Congo-Crimean hemorrhagic fever (CCHF) in Ukraine were characterized by their geographical factors. The circulation of CCHF virus was found to occur mainly in forest and forest-steppe zones irrespective of the location of administrative regions. The territories found to be most favorable for the prolonged existence of the focus of CCHF were those having high humidity.

[Cholera prevalence worldwide and in Ukraine].

In the course of the 7th pandemic cholera morbidity has been registered in 163 countries of the world. 5 periods in the development of the pandemic hav been established. The pandemic process has a pronounced tendency to growth.

[Vibrio cholerae 01, variant eltor, in the environment].

The possibilities of the autochthonous existence of V. cholerae in open water reservoirs, depending on the combined effect of different biotic and abiotic factors are considered. The role of adaptive variability of V.

[Factors contributing to preservation of Vibrio cholerae in water reservoirs].

The summarized data of literature concerning the survival of V. cholerae in the environment and the influence of abiotic and biotic factors on this process are presented. These data make it possible to regard cholera as sapronosis and to form an idea of the role of factors contributing to the survival of V.

[Antibiotic sensitivity of Vibrio cholerae 01 isolated from humans].

Antibiotic susceptibility of 1479 Vibrio cholerae 01 strains isolated from humans between 1991 and 1995 was studied. The antibiotics used belonged to different chemical groups. The assay method was that of serial dilutions in solid media.

[The evaluation of the virulent properties of Vibrio cholerae O1 by using in-vitro methods].

The virulent properties of V. cholerae O1 (more than 1000 strains) were studied. For this purpose the following methods were used: the determination of hemolytic activity, the express method based on the determination of the speed of mannite oxidation, complex analysis and polymerase chain reaction (PCR).

[Phagovars of halophilic vibrios].

The results of the phage typing of 164 halophilic vibrios revealed the most frequent combinations of lysing monophages, denoted by numbers: 1 (A, B, C, D), 2 (B, C, D), 3 (B, C) and 4 (C). In accordance with the proposed scheme, the phage typing of 398 cultures from different ecosystems was carried out. Phagovar was determined in 77.

[The variability of the biological characteristics of Vibrio cholerae isolated from environmental objects].

The study of the biological characteristics used for the identification of the species, biovar and serovar of V.cholerae O1 isolated from environmental objects on the territory of 8 regions of Ukraine for the period of 1974-1993 revealed the tendency towards an increase in the number of altered cultures. Phage sensitivity (60.

[Dynamics of changes in antibiotic sensitivity of Vibrio cholerae 01, isolated from environmental objects].

The analysis of the dynamics of the antibiotic susceptibility of 442 strains of V. cholerae 01 isolated within 1986-1994 from the environment showed that the susceptibility level was different. Strains of V.

[Antibiotic sensitivity of Vibrio cholerae 01, isolated in the Ukraine in 1994].

One thousand and four hundred strains of V. cholerae 01 isolated in 1994 in the Ukraine were studied with respect to their antibiotic susceptibility and resistance. The study showed that it was possible not only to estimate the present tendencies in and the regularities of the change in their character but also to presuppose the probable circulation and incidence of the microbe based on the differences in the susceptibility, frequency and resistance pattern of the strains of V.

[A possible mechanism for the endemicity of modern cholera (the role of noncultivated forms of Vibrio cholerae 01)].

The surface water sources of some CIS territories have been screened for cholera toxin genes by the polymerase chain reaction. The vct-genes have been found in the majority of water samples indicating the presence of noncultivated vibrio cholerae cells of an epidemiologic significance. The bacteriological methods failed to isolate the active causative agent of cholera.

[The microbiology of halophilic vibrios].

The current literature data and results of the authors' studies on the taxonomy and biological characteristics of halophilic Vibrio such as V. parahaemolyticus and V. alginolyticus are presented.

[The comparative characteristics of the antibiotic sensitivity of psychrophilic and mesophilic aeromonads].

The general regularities of the antibiotic susceptibility of psychrophilic and mesophilic aeromonads were determined. The antibioticograms were in general similar. Still, there was observed a higher susceptibility of Aeromonas salmonicida to tetracycline, chloramphenicol and rifampicin, as well as a larger number of strains susceptible to semisynthetic broad spectrum penicillins (ampicillin and carbenicillin) and cephazoline.

[The lysogeny of Vibrio parahaemolyticus of serovar O4:K12].

Lysogeny has been first established in strains of parahemolytic vibrios of serovar O4:K12. Moderate phages belonged to morphological group IV by home A. S.

[Sensitivity of Vibrio and Aeromonas to antibiotics].

The antibiotic sensitivity of 696 cultures belonging to the family Vibrionaceae (V. cholerae O1, V. cholerae non-O1, V.

[Antibiotic sensitivity of Vibrio cholerae 01 isolated from environmental objects].

The sensitivity of 252 Vibrio cholerae-O1 strains isolated from environmental objects to antibiotics of various groups was assayed by the method of serial dilutions on solid media. The biological characteristics of the isolates are presented. The Vibrio cholerae isolates with serological variation were the most frequent (36.

[The phages of halophilic vibrios and their use].

The range of the lytic activity of 46 phages of parahemolytic vibrios isolated from lysogenic strains, sea water samples, crabs and mussels has been studied. The phages are represented by virions belonging to morphological groups II, IV, V according to the phage classification currently used in Russia and to different serological groups. No relationship between the sensitivity of vibrio strains to the phages under study and the specificity of serotypes O and K has been established.

[The use of micromethods for the identification of Vibrios].

The authors recommend micromethods for laboratory studies of Vibrio; such methods may be widely used at bacteriologic laboratories for examinations of biochemical characteristics of these microorganisms, for rapid identification of V. cholerae 01, and for serologic identification (typing) of V. cholerae non 01, since they accelerate the diagnosis and are much simpler than macromethods.

[Vibrio and aeromonad sensitivity to the vibriostatic O 129].

Sensitivity of vibrios and aeromonads to vibriostatic O129 (2,4-diamino-6,7-diisopropyl pteridine) was studied. The vibrios and aeromonads were isolated in the USSR. The tests were performed with disks made in the USSR and Great Britain.

Serological diagnosis of diarrhoea caused by some representatives of the genera Vibrio and Aeromonas.

Various serological methods were used to study 630 blood sera of patients with diarrhoea on whom microorganisms of the genera Vibrio (vibrios of non-01 group, phosphorescent, parahaemolytic and alginolytic vibrios) and Aeromonas were isolated, and also of patients with unconfirmed bacteriological diagnosis, carriers and healthy persons. It has been established that specific antibodies are isolated in 30-50% of cases, most frequently in the agglutination reaction, in PHAR with flagellar erythrocyte diagnostic agent, in the reaction of vibriocidal antibodies using the Takachi apparatus. Serological diagnosis is a subsidiary method promoting the performance of clinico-epidemiological analysis of diseases with diarrhoea.