[Assessment of relative biological effectiveness of tritium using chromosome aberration frequency in human blood lymphocytes].

The aim of this work is to determine Relative Biological Effectiveness (RBE) of tritium beta-irradiation using chromosome aberration frequency in peripheral blood lymphocytes after radiation exposure in vitro and in vivo. The results of the experimental estimation of tritium beta-irradiation RBE in comparison with 60Co gamma-irradiation using analysis of unstable chromosome aberration frequencies in peripheral blood lymphocytes in reference to concrete conditions of the investigation were presented. It was demonstrated that tritium beta-irradiation is in total more effective than gamma-irradiation up to 1 Gy.

[Cytogenetic examination of nuclear specialists exposed to chronic beta-radiation of tritium].

For the first time the cytogenetic examination of the group of nuclear specialists (79 persons) chronically exposed to tritium beta-radiation for a long period was carried out. The frequencies of unstable (conventional method) and stable (FISH-method) chromosome aberrations have been analyzed. 50 years after the beginning of working under the conditions of the increased radiation level the differences (in comparison with control values) were revealed for all cytogenetic parameters.

[The analysis of mutations at mini- and microsatellite DNA loci in the family members of a group of occupationally exposed to tritium and tritium oxide plant workers].

Mini/microsatellite (MNS/MCS) loci are efficient tools in solving basic and applied problems in different spheres of biology and medicine due to their unique characteristics - a high frequency of tandem repeats in combination with their wide variability. Specifically, they have been found use as potential markers of genetic effects of ionizing radiation on animals and on human. However there is no general agreement as to the influence of irradiation on the frequency of mutations in hypervariable repetitive DNA sequences up to now.

[Study of reparative DNA synthesis in lymphocytes of persons occupationally exposed to radiation].

The UDS efficiency in lymphocytes of professionals chronically exposed to gamma/neutron radiation, as well as for a control cohort was estimated. A credible reduction of UV-induced UDS (KUV) index as compared to the control was demonstrated. This shows an invalid repair state of blood cells in professionals.

[Cytogenetic effect of small doses of tritium beta irradiation].

The effect of tritium beta-radiation within the dose range of 0.5 x 10(-3) = 0.6 Gy on human blood lymphocytes in vitro was investigated.

[Effect of pulse and prolonged x-rays in air and in nitrogen on the frequency of sex-linked recessive lethal mutations in Drosophila melanogaster].

The effect of X-irradiation on the frequency of recessive lethal mutations in Drosophila melanogaster sperm cells is studied. Drosophila males were X-irradiated in air and nitrogen in three units with doses rates of 4 X 10(9), 10(11) and 1.7 rad/sec at a dose of 2000 rad.