[The influence of mesenchymal stem cell transplantation time on myocardial reparation in rat experimental heart failure].

Mesenchymal stem cells (MSCs) have an ability to migrate in the organism to injured tissue to exert influence on inflammation and reparation in these regions. The aim of this study was to determine the optimal time of MSCs transplantation for myocardial reparation in rat experimental heart failure. The experiments were carried out on inbred line Wistar-Kyoto rats.

[Mesenchymal stem cell transplantation for myocardial reparation of rat experimental heart failure].

Mesenchymal stem cells (MSC) are resident pluripotent cells of bone marrow stroma. MSC have the ability to differentiate into osteoblasts, chondroblasts and adipocytes, neurons, glia and also into cardiomyocytes. The problem of MSC use in cell therapy of various diseases and in myocardial infarction therapy is widely discussed at present.

[Elevated expression of argentophilic proteins from the nucleolar organizer regions in myocardium of patients with obstructive hypertrophic cardiomyopathy and mutations in p53 tumor suppressor gene].

Silver staining for nucleolar proteins was used to evaluate the ribosomal genes activity in cardiomyocytes (Cm) and fibroblast-like cells (FBS) of intraventricular septum, and other regions of the left ventricle. Specimens to be analysed were taken from 7 patients with idiopathic obstructive hypertrophic cardiomyopathy (OHCM). In this group the quantity of nucleoli and AgNORs, reflecting the transcriptional and processing level of pre-ribosomal RNA in all type of cells, was at least 2 and 3 times higher than in patients with essential hypertension and in healthy control, respectively.

AgNORs in cardiomyocytes from surgical patients with coronary heart disease.

Aim: To evaluate the interphase ribosomal RNA cistron activity of cardiomyocytes in surgical patients with chronic ischaemic heart disease by means of the nucleolar organiser region silver staining (AgNOR) technique.

Methods: Nucleoli were investigated in myocardial samples obtained from 46 patients with chronic ischaemic heart disease before, during, and soon after cardioplegia ischaemia. Cryostat sections of 10 microns thickness were air dried, fixed in methanol/glacial acetic acid (3:1) for 15 minutes, rinsed carefully with distilled water, incubated in 2 N formic acid for 10 minutes, and impregnated with silver colloid solution for 2.

[Cardiac involvement in diphtheria today in adults].

Postmortem morphological examination of 102 cases with diphtheria who had died on the disease day 1-120 was performed. All the dead patients were adults. Dystrophic-necrotic processes in cardiac conduction and intramural nervous systems, in contractile myocardium developing on the disease day 1-8 were found.

[Hyperlipidemia as a factor of myocardial damage (experimental study)].

To elucidate the assumed direct negative effect of huperlipidemia (HL) on the myocardium, histological and electron microscopic studies of the myocardia of rabbits with alimentary hypercholesterolemia which were on an atherogenic diet for 30 days and underwent transesophageal pacing tests were conducted. An experimental group comprised 7 rabbits with HL, a control one included 4 animals. In HL rabbits, the cardiac pacing test caused irreversible myocardial histomorphological changes.

[An evaluation of the plastic function of the cardiomyocytes by silver staining of the nucleoli in patients operated on for ischemic heart disease].

Examinations of plastic function changes in myocardial cells (MC) from 36 patients with chronic ischemic heart disease were carried out before, during and soon after cardioplegic ischemia. The initial mean number of silver grains in nucleoli varied greatly showing some difference between groups of the patients with (9.5 +/- 0.

[Activity of the nucleolar organizers in cardiomyocytes of patients with arterial hypertension of varying genesis].

A comparative study of the myocyte nucleolar organizer activity (NOA) was performed on silver-stained myocardium from 6 patients who had died from the hypertension disease and 7 others patients with secondary renal hypertension non-complicated by severe coronary atherosclerosis and heart failure. In the first group, positive correlations between NOA of cardiac cells and the level of maximal diastolic pressure (r = 0.8, p less than 0.

[Orthostatic hypotension in primary amyloidosis].

Proceeding from clinicoanatomical observation of a 50-year-old man, the authors discuss the pathogenesis of arterial hypotension developing in generalized amyloidosis with severe affection of the cardiovascular and nervous system, and the adrenal glands.

[Characteristics of myocardial hypertrophy in arterial hypertension of various origins and in atherosclerosis].

Sets of conventional macroscopic and up-to-date histochemical techniques were employed to study hearts from 267 patients who had died of essential hypertension, atherosclerosis or secondary renal hypertension. Excessive cardiac mass was found disease-specific and related to the time since hypertension onset. Cardiac hypertrophy was augmenting with growing deficiency of coronary blood supply and showed specific correlation between cardiomyocyte nucleus and cytoplasm within each nosological unit mentioned.

[2 cases of temporal arteritis].

Two cases of temporal arteritis are described. In patient K., aged 79 years, the disease ran an acute course and resulted in bilateral blindness.

[Stereological characteristics and enzymatic activity of myocardial capillaries in different variants of pathology and death (data from immediate autopsies)].

Parallel stereo- and cytospectrophotometric examinations of human myocardial capillaries, 20-60 min after biological death were carried out. The activity of alkaline phosphatase, adenosine triphosphatase, lactate dehydrogenase and NAD-diaphorase in the capillary wall in relation to the sex and age in cardiovascular pathology, renal diseases and leukemias were studied. The permeability and level of energy supply of transendothelial transport were found to depend on the kind of the main pathological process and type of death.

[Quantitative histoenzymological and biochemical studies of the stages of atherogenesis in human coronary arteries (based on the data of early autopsies].

Histochemical methods were used to study the dynamics of activity of energetic metabolism enzymes (EME) in cell elements of the human coronary artery (CA) wall in comparison with biochemical values of lipid metabolism in the plasma. The development of CA atherosclerosis was shown to be determined to a large extent by reciprocal relations of antiatherogenic alpha-lipoproteins and atherogenic beta-lipoproteins in their influence on EME activity. An increased EME activity at early stages of atherogenesis (lipid plaque) is an adaptation to changed homeostasis.