[Comparison of two phenotypic methods for detecting the production of extended-spectrum beta-lactamases by hospital Klebsiella pneumoniae strains].

In the period 2006-2008, the Republican Emergence Medical Care Center.isolated 2997 enterobacteria, 48.9% of which belonged to Klebsiella pneumoniae.

[Experimentally determined safety and immunological activity of vaccine based on antigens isolated from Pseudomonas aeruginosa in medium K-4].

The safety and immunological activity of P. aeruginosa vaccine were experimentally evaluated. The vaccine was prepared on the basis of the antigens of P.

[Species affiliation of staphylococci isolated in purulent infections].

The microflora was studied of the clinical material taken from those patients presenting with different pyo-septic diseases. It has been ascertained that a key role in the etiology of the above diseases must be referred to gramme-positive micro-organisms--to coagulase-negative staphylococci among their number. Of these, S.

[Modified method of inoculation of hemocultures from patients with suppurative-septic diseases and typhoid-paratyphoid fever].

A modified method of isolating hemocultures in pyoseptic processes is proposed. It consists in combined use of two known methods: inoculation of the blood by in-depth method and preliminary hemolysis of the blood, and use of commercial medium for assessing the sterility as the nutrient base. High efficacy of the proposed method has been demonstrated in experiments with 17 reference strains of microorganisms.

[Hospital strains of Pseudomonas aeruginosa in the surgical clinic].

Circulation of different antigenic variants of P. aeruginosa in a surgical hospital was studied. In this study the process leading to the formation of pathogenic hospital strains, determined by time and location, from some serovars is demonstrated.

[Improving the clinico-microbiological diagnosis and treatment of non-clostridial anaerobic peritonitis].

On the basis of clinico-microbiological examinations the presence of anaerobic flora was found in 104 out of 198 patients with peritonitis. The authors propose using intra-aortal++ transfusions of oxygenated blood in complex therapy of anaerobic peritonitis.

[The role of non-clostridial anaerobic infections in the etiology, pathogenesis, clinical aspects and treatment of inflammatory diseases of the paranasal sinuses].

This paper presents the results of clinical and bacteriological examinations of 116 patients with suppurative rhinosinusitis. Bacteriological tests under anaerobic conditions revealed in 53% of the patients nonclostridium anaerobic bacteria that were highly resistant to most antibiotics and sensitive to metronidazole and its derivatives. A protocol of conservative therapy of suppurative rhinosinusitis was developed.

[Seasonal changes in the frequency of isolating strict anaerobes in purulent-inflammatory processes].

Seasonal changes in the isolation rate of obligate anaerobes from the pathological material of patients with purulent inflammatory diseases were studied. For this purpose 707 samples of pathological material were analysed in the course of 1982-1986. Anaerobes were detected in 160 samples, which constituted 22.

[Improving the microanaerostat model MI-752 and manufacturing simple microanaerostatic devices for cultivation of anaerobes].

The improvements in the design of the MI-752 microanaerostat, manufactured in this country, are aimed at improving its security. These microanaerostats are recommended to be supplied with seamless cases of various volumes or the rigidity of the case fragments attachment be strengthened with bolts. Unsophisticated devices, high-volume anaerostat and ultramicroanaerostat, are suggested.

[Microbiological diagnosis of pyo-inflammatory processes caused by strictly anaerobic microorganisms].

Basing on the literature data and their own findings, the authors have attempted a rational arrangement of the microbiologic diagnosis of anaerobic infections. The precultural and cultural stages of the diagnosis have been singled out. A hypothetic diagnosis is put forward at stage I; it is based on the sum of the clinical symptoms of anaerobic infections and on the microscopy of the native material.

[Antibiotic sensitivity of nonfermenting gram-negative bacteria].

Antibiotic resistance of 132 strains of nonfermenting gram-negative bacteria (NGNB) was studied. 43, 20, 17, 14 and 12 of them belonged to Acinetobacter calcoaceticus (anitratus and lwoffi), Pseudomonas cepacia, Alcaligenes faecalis, P. stutzeri and P.

[Sensitivity of clinical strains of facultatively anaerobic bacteria to antimicrobial drugs].

Six hundred and sixty five samples of clinical materials from patients with various pyoinflammatory diseases were tested for obligatory anaerobes. Anaerobes were detected in 148 samples which amounted to 22.3 per cent of the total number of the samples and to 33.

[Method for demonstrating strict anaerobes in microbial associations].

A procedure for indicating strict anaerobes in microbial associations was developed. The indication is performed with two disks: aminoglycoside (amikacin, 10-micrograms disk) (A) and metronidazole aminoglycoside (metronidazole + amikacin, 5- and 10-micrograms disks respectively) (MA). Colonies growing in zone A and absent in zone MA are considered to be strictly anaerobic.

[Antibiotic resistance of clinical strains of Pseudomonas aeruginosa from various O-serogroups].

The results of the study on antibiotic resistance of 745 strains of P. aeruginosa isolated in hospitals from surgical patients, environment and carriers are presented. 89.

[Characteristics of hospital strains of Pseudomonas aeruginosa].

A total of 745 P. aeruginosa strains from patients with purulent inflammatory processes, 216 strains from the environment of a surgical hospital and 35 strains from carriers were studied with respect to 30 cultural and biochemical signs of P. aeruginosa.

[Aerobic and facultatively anaerobic bacteria isolated from surgical infection patients].

The ecological structure of infections in surgical patients with different postoperative complications (suppurative wounds, abscesses, peritonitis, etc.) and suppurative lung diseases were studied. During the period of 6 years (1975-1980) 5401 samples of clinical material were studied.

[Sensitivity of clinical Ps. aeruginosa strains to antibiotics].

The results of the study on the antibiotic sensitivity of clinical strains of Ps. aeruginosa are presented. The strains were isolated from the clinical material of surgical patients with postoperative infections due to Ps.