[Induction of protein synthesis in mammalian lymphocytes during hyperthermia].

Induction of heat shock proteins (HSP's) synthesis in human lymphocytes in vitro was studied using 1D- and 2D-polyacrylamide gel electrophoresis. High resolution of the O'Farrell's procedure revealed the existence of several isoforms of HSP70. The same major HSP's (70 kDa and 88 kDa) were found in rabbit lymphocytes in vivo under whole body hyperthermia.

[Molecular mechanisms of adaptation to hyperthermia in higher organisms. III. Induction of heat-shock proteins in two Leishmania species].

The heat-shock proteins (hsp) induction in two species of Leishmania have been investigated. The species studied are parasites of two species of lizards (Lymnodactylus caspins and Agama caucasica) differing by temperature of correspondent ecological niche. Our results show that Leishmania species restricted to high-temperature host (Agama) is capable to synthesize its proteins at extreme temperatures (38, 40 degrees C) with greater intensity.

[Circular dichroism of cAMP-dependent protein kinase from pig brain].

The circular dichroism spectra of phosphorylated and non-phosphorylated forms of cAMP-dependent protein kinase from pig brain and those of the catalytical and regulatory subunits of the enzyme were studied. The percentage of the secondary structure components of the subunits was calculated. cAMP was shown to cause conformational changes of the enzyme.

[Cyclic AMP-dependent pig brain protein kinase: subunit structure, mechanism of autophosphorylation and holoenzyme dissociation under cyclic AMP action].

Some properties of cyclic AMP-dependent pig brain protein kinase were studied. The holoenzyme was shown to exist in solution in the form of a tetramer complex R2C2 with mol. weight of 180 000.