Publications by authors named "Kexin An"
Article Synopsis
- GCN5 is a histone acetyltransferase that interacts with CAMTA2 to regulate wheat grain size and weight through the activation of starch biosynthesis genes.
- gcn5 mutant grains are smaller and have less starch, while camta2 mutants show similar grain size reductions.
- The study also identifies a CAMTA2-AH3 elite allele associated with increased grain size, suggesting a potential target for breeding improved wheat varieties.
Article Synopsis
- Plant protein phosphatase 2Cs (PP2Cs) are key inhibitors in protein kinase cascades that influence plant development and response to abiotic stress.
- A genome-wide study identified 117 putative PP2C genes, which were categorized into 13 subfamilies based on phylogenetic analysis, and highlighted similar structures and conserved motifs within subfamilies.
- Key findings showed that specific CqPP2C genes from subfamily A are responsive to salt and drought stress, and they interact with various subclasses of CqSnRK2s, indicating their important role in regulating ABA signaling and stress responses in quinoa.
Ethylene plays an important role in plant development and stress resistance. The rate-limiting enzyme in ethylene biosynthesis is 1-aminocyclopropane-1-carboxylic acid synthase (ACS). () is an important food crop known for its strong tolerance to abiotic stresses.
View Article and Find Full Text PDFCRISPR/Cas9-mediated homology-directed recombination is an efficient method to express target genes. Based on the above method, providing ideal neutral integration sites can ensure the reliable, stable, and high expression of target genes. In this study, we obtained a fluorescent transformant with neutral integration and high expression of the expression cassette from the constructed expression library and named strain FS.
View Article and Find Full Text PDFThe clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas9) system is a powerful genome editing tool that has been successfully established in some filamentous fungi due to its high flexibility and efficiency. However, the potential toxicity of Cas9 restricts the further popularization and application of this system to some degree. The AMA1 element is a self-replicator derived from Aspergillus nidulans, and its derived vectors can be readily lost without selection.
View Article and Find Full Text PDFEstablishment of High-Efficiency Screening System for Gene Deletion in TB01.
J Fungi (Basel)
February 2022
Article Synopsis
- * A new visualized gene deletion system was created that uses green fluorescence protein (GFP) to easily identify fluorescent deletion transformants from nonfluorescent ones with a simple hand-held lamp.
- * This new system improves the efficiency of screening transformants by about four times compared to traditional PCR methods, making it a more convenient and cost-effective option for researchers.
Article Synopsis
- Breeding efforts in wheat focus on enhancing grain yield and quality, particularly through the content and composition of seed storage proteins (SSPs) and starch levels.
- The study investigates the role of the endosperm-specific NAC transcription factor TaNAC019 in the coordination of SSP and starch accumulation, revealing that it binds to certain genes related to gluten and starch metabolism.
- The research identifies an elite allele (TaNAC019-BI) linked to improved flour processing quality, suggesting a potential target for breeding programs aimed at enhancing wheat quality.
Polyploidy occurs prevalently and plays an important role during plant speciation and evolution. This phenomenon suggests polyploidy could develop novel features that enable them to adapt wider range of environmental conditions compared with diploid progenitors. Bread wheat (Triticum aestivum L.
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