Recovery of compaction resources in internal dump of gently inclined composite coal seam open-pit mine.

In the production and construction process of a slowly inclined composite coal seam surface mine in China, the problem of coal compression by the non-working slope is common, but the research on coal recovery is usually conducted for the end slope compression problem. Due to the small economic and reasonable stripping ratio in the early stage of infrastructure construction in an open-pit mine, the buried deep coal seam has not yet been mined and is covered by internal dumping. In recent years, with the gradual increase of the economic and reasonable stripping ratio, the mining of coal seam covered by internal dumping site has become feasible.

Nitric oxide synthase and renin-angiotensin gene expression and NOS function in the postnatal renal resistance vasculature.

Nitric oxide (NO), produced by nitric oxide synthase (NOS), critically counteracts angiotensin-II-enhanced vascular resistance in the immature kidney, perhaps due to the developmental regulation of NOS expression and function in the postnatal preglomerular resistance vessels (PRV). Our experiments measured the messenger ribonucleic acid (mRNA) gene expression of neuronal NOS (nNOS), endothelial NOS (eNOS), and components of the renin-angiotensin system (renin, AT1 and AT2 receptors), by real-time RT-PCR, as well as NOS enzymatic activity by citrulline assay in PRVs (afferent, interlobular, and arcuate arterioles) obtained from swine ages newborn, 7 and 21 days, and adult. NOS enzymatic activity was upregulated in PRVs immediately after birth but decreased to adult levels with maturation.

Estrogen down-regulates GnRH gene expression in human placental cytotrophoblast cells.

This study aims at investigating and characterizing GnRH gene expression and regulation in human placental cytotrophoblasts by estradiol. RT-PCR analysis demonstrates that the cytotrophoblasts expressed the pro-GnRH gene. PCR with two pairs of oligonucleotide primers generated doublets 870 bases apart at the expected molecular weights, indicating that the upstream and downstream transcriptional start sites of the GnRH gene were utilized and that transcripts with and without intron I were both produced.