Determination of 17β-estradiol by surface-enhanced Raman spectroscopy merged with hybridization chain reaction amplification on Au@Ag core-shell nanoparticles.

The authors describe an aptamer-based assay for 17β-estradiol. It relies on the combined use of surface enhanced Raman scattering (SERS) and hybridization chain reaction (HCR). The aptamer against 17β-estradiol is applied as the recognition probes, and this results in excellent specificity.

Efficacy of sequential three-step empirical therapy for chronic cough.

Background: Empirical three-step therapy has been proved in just one hospital. This study aimed to demonstrate applicability of the sequential empirical three-step therapy for chronic cough in different clinical settings.

Methods: Sequential empirical three-step therapy was given to patients with chronic cough in one tertiary and three secondary care respiratory clinics.

Colorimetric Integrated PCR Protocol for Rapid Detection of Vibrio parahaemolyticus.

Rapid detection of pathogens is of great significance for food safety and disease diagnosis. A new colorimetric method for rapid and easy detection of ( or Vp) has been developed in this research. A specific sequence was designed and integrated with the forward primer for molecular detection of Vp.

Effectiveness of Inspiratory Termination Synchrony with Automatic Cycling During Noninvasive Pressure Support Ventilation.

BACKGROUND Pressure support ventilation (PSV) is a standard method for non-invasive home ventilation. A bench study was designed to compare the effectiveness of patient-ventilator inspiratory termination synchronization with automated and conventional triggering in various respiratory mechanics models. MATERIAL AND METHODS Two ventilators, the Respironics V60 and Curative Flexo ST 30, connected to a Hans Rudolph Series 1101 lung simulator, were evaluated using settings that simulate lung mechanics in patients with chronic obstructive pulmonary disease (COPD), acute respiratory distress syndrome (ARDS), or normal lungs.

Performance characteristics of seven bilevel mechanical ventilators in pressure-support mode with different cycling criteria: a comparative bench study.

Background: Pressure support ventilation from a bilevel device is a standard technique for non-invasive home ventilation. A bench study was designed to compare the performance and patient-ventilator synchronization of 7 bilevel ventilators, in the presence of system leaks.

Material/methods: Ventilators were connected to a Hans Rudolph Series 1101 lung simulator (compliance, 50 mL/cmH2O; expiratory resistance, 20 cmH2O/L/s; respiratory rate, 15 breaths/min; inspiratory time, 1.

[Effect on inspiratory cycling-off synchronization with different expiratory trigger sensitivity level during pressure-support ventilation: a lung model study].

Objective: To compare the inspiratory cycling-off synchronization with different expiratory trigger sensitivity (ETS) setting during noninvasive pressure support ventilation (PSV) under the conditions of system leak.

Methods: Ventilators were connected to a lung model (Hans Rudolph Series1101 simulator). And its mechanics was set in two models of obstructive and restrictive ventilatory dysfunction.

MiRNA synergistic network construction and enrichment analysis for common target genes in small-cell lung cancer.

Background: Small-cell lung cancer (also known as SCLC) is an aggressive form and untreated patients generally die within about 3 months. To obtain further insight into mechanism underlying malignancy with this cancer, an miRNA synergistic regulatory network was constructed and analyzed in the present study.

Method: A miRNA microarray dataset was downloaded from the NCBI GEO database (GSE27435).