Label-Free and Rapid Microfluidic Design Rules for Circulating Tumor Cell Enrichment and Isolation: A Review and Simulation Analysis.

Enriching and isolating circulating tumor cells (CTCs) have attracted significant interest due to their important role in early cancer diagnosis and prognosis, allowing for minimally invasive approaches and providing vital information about metastasis at the cellular level. This review comprehensively summarizes the recent developments in microfluidic devices for CTC enrichment and isolation. The advantages and limitations of several microfluidic devices are discussed, and the design specifications of microfluidic devices for CTC enrichment are highlighted.

Gut-derived macrophages link intestinal damage to brain injury after cardiac arrest through TREM1 signaling.

Brain injury is the leading cause of death and disability in survivors of cardiac arrest, where neuroinflammation triggered by infiltrating macrophages plays a pivotal role. Here, we seek to elucidate the origin of macrophages infiltrating the brain and their mechanism of action after cardiac arrest/cardiopulmonary resuscitation (CA/CPR). Wild-type or photoconvertible Cd68-Cre:R26-LSL-KikGR mice were subjected to 10-min CA/CPR, and the migration of gut-derived macrophages into brain was assessed.

Ferroelectric Optoelectronic Memory Based on p-GaN/ZnGaO/BaTiO/n-ITO Heterojunction with Integrated Sensing and Logic Operations.

Ferroelectric optoelectronic memories, capable of integrating sensing, computing, and storage functionalities, hold significant potential in the fields of artificial intelligence and the Internet of Things. In this study, a nonvolatile p-GaN/ZnGaO/BaTiO/n-ITO ferroelectric optoelectronic memory is demonstrated. By combining with wide-bandgap semiconductors ZnGaO and GaN, known for their excellent optoelectronic properties, the device exhibits superior self-powered ultraviolet photodetection performance.

Decorated-Induced Oxygen Vacancy Engineering for Ultra-Low Concentration Nonanal Sensing: A Case Study of La-Decorated BiOCO.

La-decorated BiOCO (BCO-La) microspheres are synthesized using a facile wet chemical strategy for sensing low-concentration nonanal (CHO) at room temperature. These BCO-La gas sensors are applied to evaluate agricultural product quality, specifically for cooked rice. The sensitivity of the BCO-6La sensor significantly surpassed that of the pure BCO sensor, achieving a response value of 174.

Intravenous thrombolysis versus dual antiplatelet therapy in minor ischemic stroke within the thrombolytic window (TAMIS): a multicenter cohort study.

Intravenous thrombolysis (IVT) and dual antiplatelet therapy (DAPT) have been widely used in minor ischemic stroke (MIS) treatment. However, the clinical outcomes and safety of these two treatments have not been compared within the early thrombolytic time window. Here, we conducted a multicenter, ambispective cohort study involving patients with MIS presenting within 4.

CuFeO/lignin-based carbon composited photothermal and photodynamic agents for synergetic antibacterial therapy.

Bacterial infection has become the second leading cause of death in the world. Exploring a new highly antibacterial catalyst to replace traditional antibacterial agent is crucial for the society development of human beings. In this study, CuFeO/Lg-based carbon composited catalysts were rationally constructed by facile hydrothermal method.

Stabilization of TGF-β Receptor 1 by a Receptor-Associated Adaptor Dictates Feedback Activation of the TGF-β Signaling Pathway to Maintain Liver Cancer Stemness and Drug Resistance.

Dysregulation of the transforming growth factor-β (TGF-β) signaling pathway regulates cancer stem cells (CSCs) and drug sensitivity, whereas it remains largely unknown how feedback regulatory mechanisms are hijacked to fuel drug-resistant CSCs. Through a genome-wide CRISPR activation screen utilizing stem-like drug-resistant properties as a readout, the TGF-β receptor-associated binding protein 1 (TGFBRAP1) is identified as a TGF-β-inducible positive feedback regulator that governs sensitivity to tyrosine kinase inhibitors (TKIs) and promotes liver cancer stemness. By interacting with and stabilizing the TGF-β receptor type 1 (TGFBR1), TGFBRAP1 plays an important role in potentiating TGF-β signaling.

Room-Temperature Sensing Mechanism of GQDs/BiSbO Nanorod Clusters: Experimental and Density Functional Theory Study.

Creating high-performance gas sensors for heptanal detection at room temperature demands the development of sensing materials that incorporate distinct spatial configurations, functional components, and active surfaces. In this study, we employed a straightforward method combining hydrothermal strategy with ultrasonic processing to produce mesoporous graphene quantum dots/bismuth antimonate (GQDs/BiSbO) with nanorod cluster forms. The BiSbO was incorporated with appropriate contents of GQDs resulting in significantly improved attributes such as heightened sensitivity (59.

Combination of Dasatinib and Quercetin alleviates heat stress-induced cognitive deficits in aged and young adult male mice.

Objective: Dasatinib and quercetin (D & Q) have demonstrated promise in improving aged-related pathophysiological dysfunctions in humans and mice. Herein we aimed to ascertain whether the heat stress (HS)-induced cognitive deficits in aged or even young adult male mice can be reduced by D & Q therapy.

Methods: Before the onset of HS, animals were pre-treated with D & Q or placebo for 3 consecutive days every 2 weeks over a 10-week period.

IL-33 Accelerates Chronic Atrophic Gastritis through AMPK-ULK1 Axis Mediated Autolysosomal Degradation of GKN1.

Chronic atrophic gastritis (CAG) is a complex disease characterized by atrophy and inflammation in gastric mucosal tissue, especially with high expression of interleukins. However, the interaction and mechanisms between interleukins and gastric mucosal epithelial cells in CAG remain largely elusive. Here, we elucidate that IL-33 stands out as the predominant inflammatory factor in CAG, and its expression is induced by and MNNG through the ROS-STAT3 signaling pathway.

Diagnostic accuracy of oral swab for detection of pulmonary tuberculosis: a systematic review and meta-analysis.

Objectives: Tuberculosis (TB) remains a significant concern in terms of public health, necessitating the timely and accurate diagnosis to impede its advancement. The utilization of oral swab analysis (OSA) presents a promising approach for diagnosing pulmonary TB by identifying (MTB) within oral epithelial cells. Due to disparities in the diagnostic performance of OSA reported in the original studies, we conducted a meticulous meta-analysis to comprehensively assess the diagnostic efficacy of OSA in pulmonary TB.

LC-based lightfield camera prototype for rapidly creating target images optimized by finely adjusting several key coefficients and a LC-guided refocusing-rendering.

A lightfield camera prototype is constructed by directly coupling a liquid-crystal (LC) microlens array with an arrayed photosensitive sensor for performing a LC-guided refocusing-rendering imaging attached by computing disparity map and extracting featured contours of targets. The proposed camera prototype presents a capability of efficiently selecting the imaging clarity value of the electronic targets interested. Two coefficients of the calibration coefficient k and the rendering coefficient C are defined for quantitively adjusting LC-guided refocusing-rendering operations about the images acquired.

Integrated microcavity electric field sensors using Pound-Drever-Hall detection.

Discerning weak electric fields has important implications for cosmology, quantum technology, and identifying power system failures. Photonic integration of electric field sensors is highly desired for practical considerations and offers opportunities to improve performance by enhancing microwave and lightwave interactions. Here, we demonstrate a high-Q microcavity electric field sensor (MEFS) by leveraging the silicon chip-based thin film lithium niobate photonic integrated circuits.

Association between the intake of dietary n3 and n6 fatty acids and stroke in US adults: A cross-sectional study of NHANES 2007-2018.

Background: The association between the intake of dietary n3 and n6 fatty acids and the risk of stroke is subject to debate. The primary objective of the present research was to establish the correlation in a large sample of American adults.

Methods: Using data from the National Health and Nutrition Examination Survey (NHANES) between 2007 and 2018, the association of the intake of dietary n3 and n6 fatty acids with stroke events was analyzed in a sample of 29,459 adults.

Spatiotemporal mode-locking and dissipative solitons in multimode fiber lasers.

Multimode fiber (MMF) lasers are emerging as a remarkable testbed to study nonlinear spatiotemporal physics with potential applications spanning from high energy pulse generation, precision measurement to nonlinear microscopy. The underlying mechanism for the generation of ultrashort pulses, which can be understood as a spatiotempoal dissipative soliton (STDS), in the nonlinear multimode resonators is the spatiotemporal mode-locking (STML) with simultaneous synchronization of temporal and spatial modes. In this review, we first introduce the general principles of STML, with an emphasize on the STML dynamics with large intermode dispersion.

Integrated bioinformatics analysis of dendritic cells hub genes reveal potential early tuberculosis diagnostic markers.

Background: Dendritic cells (DCs) are most potent antigen-processing cells and play key roles in host defense against Mycobacterium tuberculosis (MTB) infection. In this study, hub genes in DCs during MTB infection were first investigated using bioinformatics approaches and further validated in Monocyte-derived DCs.

Methods: Microarray datasets were obtained from Gene Expression Omnibus (GEO) database.

Ketogenic diet and β-Hydroxybutyrate alleviate ischemic brain injury in mice via an IRAKM-dependent pathway.

Ketogenic diet (KD) is a classical nonpharmacological therapy that has recently been shown to benefit cerebral ischemia, but the mechanism remains unclear. This study investigated the neuroprotective effects of KD pretreatment and β-hydroxybutyrate (BHB, bioactive product of KD) post-treatment in a mouse model of temporary middle cerebral artery occlusion (tMCAO). Neurological function, infarct volume, as well as inflammatory reactions are evaluated 24 h after ischemia.

IL-35 Stabilizes Treg Phenotype to Protect Cardiac Allografts in Mice.

Background: Interleukin-35 (IL-35), secreted by regulatory T cells (Treg) and B cells, is immunosuppressive under both physiological and pathological conditions. However, the role of IL-35 in all responses has yet to be investigated. Here, we demonstrate that IL-35 protects allografts by stabilizing the Treg phenotype and suppressing CD8 + T-cell activation in a mouse heart transplantation model.

Diploid and tetraploid genomes of Acorus and the evolution of monocots.

Monocots are a major taxon within flowering plants, have unique morphological traits, and show an extraordinary diversity in lifestyle. To improve our understanding of monocot origin and evolution, we generate chromosome-level reference genomes of the diploid Acorus gramineus and the tetraploid Ac. calamus, the only two accepted species from the family Acoraceae, which form a sister lineage to all other monocots.

Highly sensitive and selective SbWO microspheres in detecting VOC biomarkers in cooked rice: Experimental and density functional theory study.

The palatability of cooked rice is susceptible to the flavor and effective detection of volatile organic compounds (VOCs) can avoid deterioration and improve the taste quality. Herein, hierarchical antimony tungstate (SbWO) microspheres are synthesized through a solvothermal process and the effect of solvothermal temperature on the room temperature gas-sensing properties of gas sensors is investigated. Outstanding sensitivity towards VOC biomarkers (nonanal, 1-octanol, geranyl acetone and 2-pentylfuran) in cooked rice is achieved and the sensors exhibit remarkable stability and reproducibility, which are contributed to the formation of the hierarchical microsphere structure, larger specific surface area, narrower band gap and increased oxygen vacancy content.