Towards a more objective and high-throughput spheroid invasion assay quantification method.

Multicellular spheroids embedded in 3D hydrogels are prominent in vitro models for 3D cell invasion. Yet, quantification methods for spheroid cell invasion that are high-throughput, objective and accessible are still lacking. Variations in spheroid sizes and the shapes of the cells within render it difficult to objectively assess invasion extent.

Towards a More Objective and High-throughput Spheroid Invasion Assay Quantification Method.

Multicellular spheroids embedded in 3D hydrogels are prominent models for 3D cell invasion. Yet, quantification methods for spheroid cell invasion that are high throughput, objective and accessible are still lacking. Variations in spheroid sizes and the shapes of the cells within render it difficult to objectively assess invasion extent.