Fe-Co-Fe prussian blue analogues loaded nitrogen doped carbon quantum dots for effective epinephrine detection.

Epinephrine (Ep) is an important neurotransmitter, which plays an important role in the nervous system and glycogen metabolism of living organisms. Hence, a novel NCQDs/FeCoFe-PBA composite with FeCoFe-Prussian blue analogues (PBA) as the core and nitrogen-doped carbon quantum dots (NCQDs) as the shell was constructed by a one-pot hydrothermal method, and it was used for the efficient detection of Ep. As a good electroactive material, NCQDs in the composite not only improved the weak conductivity of FeCoFe-PBA, but also limited the self-aggregation of FeCoFe-PBA, and formed a uniform shell on FeCoFe-PBA.

Development of CuFeO microspheres/carbon sheets composite materials as a sensitive electrochemical sensor for determination of bisphenol A.

A composite material based on CuFe-ZIF-derived CuFeO nano-microspheres grown in situ and well-ordered on carbon sheets (CS) was prepared and applied for highly effective determination of bisphenol A (BPA). The composite material possessed inherently high redox activity due to the presence of both Cu and Fe ions with various oxidation states (Cu²⁺/Cu⁺ and Fe³⁺/Fe²⁺), high specific surface area, uniform distribution of Cu and Fe ions, and a robust framework imparted by its precursor CuFe-ZIF. This led to increased active sites for electrochemical reactions, improved electron transfer efficiency, and structural integrity during electrochemical cycling.

NURD 2.0: Prediction of tibial nonunion after intramedullary nail fixation at any time within 3 months after injury.

Introduction Nonunion after fixation of long bones negatively impacts outcomes and requires additional surgery. The ability to predict likelihood of nonunion after tibial shaft fracture would be helpful to clinicians and patients. The goal of this work was to combine three previous models of tibial shaft nonunion at different time points into one overall model that incorporates time as a continuous variable.

Predicting tibia shaft nonunions at initial fixation: An external validation of the Nonunion Risk Determination (NURD) score in the SPRINT trial data.

Background: Predictive models are common in orthopedic research; however, most models are not validated in an external population. The Nonunion Risk Determination (NURD) score was developed using a single-center cohort of 382 patients to reliably predict tibia shaft nonunions at the time of initial intramedullary nail fixation. The purpose of this study was to externally validate the NURD score using data from the SPRINT Trial.

Prediction of tibial nonunion at the 6-week time point.

Introduction: Intramedullary (IM) nail fixation is a common operative treatment, yet concerns regarding the frequency of complications, such as nonunion, remain. Treatment of tibial shaft fractures remains a challenge, and little evidence of prognostic factors that increase risk of nonunion is available. The aim of this study was to develop a predictive model of tibial shaft fracture nonunion 6 weeks after reamed intramedullary (IM) nail fixation based on commonly collected clinical variables and the radiographic union score for tibial fractures (RUST).

Polyoxometalate-encapsulated twenty-nuclear silver-tetrazole nanocage frameworks as highly active electrocatalysts for the hydrogen evolution reaction.

Two unprecedented polyoxometalate-encapsulated twenty-nuclear silver-tetrazole nanocage frameworks have been synthesized, which exhibit high activity in hydrogen evolution reaction. HUST-100 shows an onset overpotential of 148 mV and a Tafel slope of 82 mV dec, and the catalytic current density approaches 10 mA cm at an overpotential of 234 mV.

Will My Tibial Fracture Heal? Predicting Nonunion at the Time of Definitive Fixation Based on Commonly Available Variables.

Background: Accurate prediction of tibial nonunions has eluded researchers. Reliably predicting tibial nonunions at the time of fixation could change management strategies and stimulate further research.

Questions/purposes: We asked (1) whether data from medical records, fracture characteristics, and radiographs obtained at the time of fixation would identify features predictive of tibial fracture nonunion; and (2) whether this information could be used to create a model to assess the chance of nonunion at the time of intramedullary (IM) nail fixation of the tibia.

An unprecedented 3D POM-MOF based on (7,8)-connected twin Wells-Dawson clusters: synthesis, structure, electrocatalytic and photocatalytic properties.

The first (7,8)-connected polyoxometalate-based metal-organic framework (POM-MOF) has been constructed from seven- and eight-connected twin Wells-Dawson clusters, and possesses the highest connection number of polyoxometalates to any mixed-connected POM-MOF to date and a unique structural motif that contains both organic-inorganic and all-inorganic networks.

A novel technique for reducing intertrochanteric hip fractures.

Intertrochanteric hip fractures typically become deformed by the muscular and gravitational forces acting on the 2 main bony fragments. Traditional use of a fracture table for anatomical reduction normally corrects for the varus angulation, external rotation, and posterior sag that can occur, but, in select unstable and comminuted fractures, reduction may not be possible because of posterior sag and external rotation of the proximal fragment. These aspects of malreduction have been addressed in multiple ways, including use of unscrubbed assistants, crutches, internal rotation of the distal fragment by internal rotation of the foot, bumps and pads, and even intraoperative techniques.

Comparative lower limb hemodynamics using neuromuscular electrical stimulation (NMES) versus intermittent pneumatic compression (IPC).

Deep Vein Thrombosis (DVT) is a life threatening condition and a serious concern among hospitalised patients, with death occurring in approximately 6% of cases. Intermittent pneumatic compression (IPC) is commonly used for DVT prevention, however suffers from low compliance and issues of usability and portability. Neuromuscular electrical stimulation (NMES) has been shown to improve lower limb hemodynamics but direct comparison with IPC in terms of hemodynamics is rare but very important to determine the potential effectiveness of NMES in DVT prevention.

The role of accountable care organizations in delivering value.

The goal of Accountable Care Organizations is to improve patient outcomes while maximizing the value of the services provided. This will be achieved through the use of performance and quality measures that facilitate efficient, cost-effective, evidence-based care. By creating a network connecting primary care physicians, specialists, rehabilitation facilities and hospitals, patient care should be maximized while at the same time delivering appropriate value for those services provided.

Revisiting the polyoxometalate-based late-transition-metal-oxo complexes: the "oxo wall" stands.

Terminal oxo complexes of the late transition metals Pt, Pd, and Au have been reported by us in Science and Journal of the American Chemical Society. Despite thoroughness in characterizing these complexes (multiple independent structural methods and up to 17 analytical methods in one case), we have continued to study these structures. Initial work on these systems was motivated by structural data from X-ray crystallography and neutron diffraction and (17)O and (31)P NMR signatures which all indicated differences from all previously published compounds.

Pseudoaneurysm of the distal thigh after manipulation of a total knee arthroplasty.

The authors describe a unique complication after manipulation of a stiff total knee arthroplasty in a 47-year-old man. Four days after undergoing manipulation under anesthesia (MUA), the patient presented with increasing pain and swelling of the affected knee and decreased hemoglobin/hematocrit. Computed tomographic angiogram revealed a ruptured pseudoaneurysm of a segmental branch of the deep femoral artery that was treated with embolization and anterior thigh compartment fasciotomy.

Synthesis and characterization of a metal-to-polyoxometalate charge transfer molecular chromophore.

[P(4)W(35)O(124){Re(CO)(3)}(2)](16-) (1), a Wells-Dawson [α(2)-P(2)W(17)O(61)](10-) polyoxometalate (POM)-supported [Re(CO)(3)](+) complex containing covalent W(VI)-O-Re(I) bonds has been synthesized and characterized by several methods, including X-ray crystallography. This complex shows a high visible absorptivity (ε(470 nm) = 4000 M(-1) cm(-1) in water) due to the formation of a Re(I)-to-POM charge transfer (MPCT) band. The complex was investigated by computational modeling and transient absorption measurements in the visible and mid-IR regions.

Development and validation of a multiplex microsphere-based assay for detection of domestic cat (Felis catus) cytokines.

Cytokines are essential signaling molecules that mediate the innate immune response, and therefore their presence can be of diagnostic, prognostic, and pathogenic significance. Microsphere-based immunoassays allow rapid and accurate evaluation of cytokine levels in several species, including humans, dogs, and mice; however, technology to evaluate domestic cat (Felis catus) cytokines has been limited to single-analyte enzyme-linked immunosorbent assays (ELISAs). Microsphere-based immunoassays provide an attractive alternative technology for detecting and quantifying multiple analytes in a single assay using as little as 50 μl of sample.

Switching slow relaxation in a Mn(III)(3)Mn(IV) cluster: an example of grafting single-molecule magnets onto polyoxometalates.

Binding an established single-molecule magnet cluster of distorted cubane type [Mn(III)(3)Mn(IV)O(4)] to the lacunary site of an {alpha-P(2)W(15)} polyoxotungstate scaffold results in a surprising zero-field splitting inversion and the subsequent loss of magnetization bistability.

Feline leukemia virus immunity induced by whole inactivated virus vaccination.

A fraction of cats exposed to feline leukemia virus (FeLV) effectively contain virus and resist persistent antigenemia/viremia. Using real-time PCR (qPCR) to quantitate circulating viral DNA levels, previously we detected persistent FeLV DNA in blood cells of non-antigenemic cats considered to have resisted FeLV challenge. In addition, previously we used RNA qPCR to quantitate circulating viral RNA levels and determined that the vast majority of viral DNA is transcriptionally active, even in the absence of antigenemia.

Utilizing the FIV model to understand dendritic cell dysfunction and the potential role of dendritic cell immunization in HIV infection.

Dendritic cells (DC) are potent antigen presenting cells which initiate and coordinate the immune response making them central targets of and attractive candidates for manipulation in chronic lentiviral infections. Emerging evidence suggests that DC immune function is disrupted during both acute and chronic infection with human immunodeficiency virus (HIV), simian immunodeficiency virus (SIV), and feline immunodeficiency virus (FIV). Despite some early promising data, the use of DC for lentiviral immunotherapy has not fulfilled its expected potential and has been complicated by the large number of variables involved in DC harvesting, purifying, and antigen loading.

Multifunctional multilayer films containing polyoxometalates and bismuth oxide nanoparticles.

Multifunctional multilayer films consisting of the Keggin-type polyoxometalate [SiW(9)V(3)O(40)](7-) (SiW(9)V(3)) and bismuth oxide nanoparticles (Bi(2)O(3)) were prepared by the layer-by-layer assembly method. For the first time, electrochromic and photochromic studies were done on a film containing both polyoxometalates and nanoparticles. The films were characterized by UV-vis absorption, emission spectra, and atomic force microscopy.

Altered bone marrow dendritic cell cytokine production to toll-like receptor and CD40 ligation during chronic feline immunodeficiency virus infection.

Impaired dendritic cell (DC) function is thought to be central to human immunodeficiency virus-associated immunodeficiency. In this study, we examined the effect of chronic feline immunodeficiency virus (FIV) infection on DC cytokine production in response to microbial and T-cell stimulation. Cytokine production after either Toll-like receptor (TLR) or CD40 ligation in bone marrow-derived DCs (BM-DCs) was measured in naïve and chronically FIV-infected cats.

Development and application of a quantitative real-time PCR assay to detect feline leukemia virus RNA.

We previously defined four categories of feline leukemia virus (FeLV) infection, designated as abortive, regressive, latent, and progressive. To determine if detectable viral DNA is transcriptionally active in the absence of antigenemia, we developed and validated a real-time viral RNA qPCR assay. This assay proved to be highly sensitive, specific, reproducible, and allowed reliable quantitation.

Feline immunodeficiency virus dendritic cell infection and transfer.

Feline immunodeficiency virus (FIV) interacts with dendritic cells (DC) during initiation of infection, but whether DC support or transfer FIV infection remains unclear. To address this issue, we studied the susceptibility of feline myeloid DC to FIV infection and assessed potential transfer of infection from DC to CD4(+) T cells. FIV was detected in membrane-bound vesicles of DC within 2 h of inoculation, although only low concentrations of FIV DNA were found in virus-exposed isolated DC.

Kinetics of early FIV infection in cats exposed via the vaginal versus intravenous route.

To determine the influence of route of virus exposure on early pathogenesis of feline immunodeficiency virus (FIV) infection, cats were exposed to either of two FIV isolates (FIV-B-2542 or FIV-A-PPR) by vaginal or intravenous (IV) inoculation. Exposure to either virus clade by either route of inoculation resulted in vaginal and systemic infection. Peak plasma viremia and tissue proviral burden were 1-3 log(10) greater in cats infected with FIV-B-2542 vs.