Minimum Selenium Requirements Increase When Repleting Second-Generation Selenium-Deficient Rats but Are Not Further Altered by Vitamin E Deficiency.

Second-generation selenium-deficient weanling rats fed graded levels of dietary Se were used (a) to study the impact of initial Se deficiency on dietary Se requirements; (b) to determine if further decreases in selenoperoxidase expression, especially glutathione peroxidase 4 (Gpx4), affect growth or gross disease; and (c) to examine the impact of vitamin E deficiency on biochemical and molecular biomarkers of Se status. Rats were fed a vitamin E-deficient and Se-deficient crystalline amino acid diet (3 ng Se/g diet) or that diet supplemented with 100 μg/g all-rac-α-tocopheryl acetate and/or 0, 0.02, 0.

Blood glutathione peroxidase-1 mRNA levels can be used as molecular biomarkers to determine dietary selenium requirements in rats.

Transcript (mRNA) levels are increasingly being used in medicine as molecular biomarkers for disease and disease risk, including use of whole blood as a target tissue for analysis. Development of blood molecular biomarkers for nutritional status, too, has potential application that parallels opportunities in medicine, including providing solid data for individualized nutrition. We previously reported that blood glutathione peroxidase-1 (Gpx1) mRNA was expressed at levels comparable to major tissues in rats and humans.

Dietary selenium requirements based on tissue selenium concentration and glutathione peroxidase activities in old female rats.

Dietary nutrient requirements for older animals have been studied far less than have requirements for young growing animals. To determine dietary selenium (Se) requirements in old rats, we fed female weanling rats a Se-deficient diet (0.007 microg Se/g) or supplemented rats with graded levels of dietary Se (0-0.

The influence of reported trauma and adverse events on eating disturbance in young adults.

Objective: Early experiences of trauma or adverse events may be associated with eating disturbance later in life, but evidence is scarce. This study examined whether reported history of adverse life events predicted eating disturbance upon college entry and prospective changes over the first semester of college.

Method: First semester college students (n = 249) reported trauma/adverse event histories and completed disordered eating questions (with two factors, restriction and binging/purging) at the beginning and end of their first semester.

Alcohol-related legal infractions and student retention.

Objective: The present study employed municipal alcohol-related arrest reports to determine if being arrested/cited reduced the probability of academic retention.

Method: Alcohol-related legal infraction data implicating 1,310 college students was gathered during a 4-year period. First- through third-year students were identified in the database by cross-checking names in the campus directory.

Chronic pain, meaning, and spirituality: A qualitative study of the healing process in relation to the role of meaning and spirituality.

This qualitative study explores experiences of individuals with chronic pain in their attempt to find meaning in the presence of continual pain. Fifteen participants at Loma Linda University Behavioral Medicine Center were interviewed. Emerging themes from this study show that (1) meaning is initially defined as the ability to engage in productive activities and positive relationships; (2) chronic pain is perceived as the factor that removes meaning from the lives of sufferers; (3) medication is used to cope with pain, leading to addiction; (4) addiction results in greater loss of meaning; and (5) rediscovery of meaning takes place through a more complex understanding of the self that embraces suffering and thus is able to explain the interrelation of pain, emotions, and addiction.

Dietary selenium requirements based on glutathione peroxidase-1 activity and mRNA levels and other Se-dependent parameters are not increased by pregnancy and lactation in rats.

The hierarchy of selenium (Se) requirements for growing rats ranges from <0.01 to 0.1 microg Se/g diet, depending on the choice of Se status parameter.

Sexual trauma, spirituality, and psychopathology.

This study assessed the association between spirituality and psychopathology in a group of sexual abuse victims and controls with a focus on whether spirituality moderated the association between sexual trauma and psychopathology. Seventy-one sexual trauma victims were compared to 25 control subjects on spiritual well-being, the Eating Disorder Examination, the PTSD Symptom Scale, and the SCID-I/P. The data showed that the two groups did not differ in terms of spiritual well-being.

Adolescent and adult alcohol attitudes in a high alcohol consumption community.

The study examines alcohol-related attitudes among adolescents and adults in a high consumption community, exploring whether adolescents and adults hold similar or different views regarding adolescent drinking. Data were gathered from adults in a Midwestern city via random telephone survey of 487 adults (30% with children under the age of 21). Students in grades 6-12 (n = 558) also completed a youth version of the survey in classrooms.

Psychopathology and sexual trauma in childhood and adulthood.

This study evaluates the occurrence of psychopathology among 97 women who (1) experienced sexual abuse in childhood only, (2) were raped in adulthood only, (3) experienced both childhood sexual abuse and rape in adulthood, or (4) experienced no sexual trauma. Women were recruited from advertisements and assessed using the Structured Clinical Interview for DSM-IV (SCID-I/P) and the Modified PTSD Symptom Scale Self-Report. Women who reported sexual trauma were significantly more likely to exhibit psychopathology than controls.

Dietary selenium regulation of glutathione peroxidase mRNA and other selenium-dependent parameters in male rats.

Weanling male rats were fed a basal torula yeast diet (0.007 μg Se/g diet) supplemented with graded levels of Se (0 to 0.2 μg Se/g diet as NaSeO) (three rats/group) to evaluate classical glutathione peroxidase (GPX1, GSH:HO, oxidoreductase, EC 1.