Management of painful temporomandibular disorders: Methods and overview of The National Dental Practice-Based Research Network prospective cohort study.

Background: Patients often seek consultation with dentists for temporomandibular disorders (TMDs). The objectives of this article were to describe the methods of a large prospective cohort study of painful TMD management, practitioners' and patients' characteristics, and practitioners' initial treatment recommendations conducted by The National Dental Practice-Based Research Network (the "network").

Methods: Participating dentists recruited into this study treated patients seeking treatment for painful TMDs.

Associations of types of pain with crack-level, tooth-level and patient-level characteristics in posterior teeth with visible cracks: Findings from the National Dental Practice-Based Research Network.

Objectives: The objective of this study was to determine which patient traits, behaviors, external tooth and/or crack characteristics correlate with the types of symptoms that teeth with visible cracks exhibit, namely pain on biting, pain due to cold stimuli, or spontaneous pain.

Methods: Dentists in the National Dental Practice-Based Research Network enrolled a convenience sample of subjects each of whom had a single, vital posterior tooth with at least one observable external crack (cracked teeth); 2858 cracked teeth from 209 practitioners were enrolled. Data were collected at the patient-, tooth-, and crack-level.

A novel, minimally invasive approach to managing mild epithelial dysplasia.

Conventional oral cancer screening examinations can be enhanced by direct tissue fluorescence visualization. Early dysplastic lesions detected during screening examinations often are monitored for progression or changes in appearance. Aggressive surgical intervention usually is contraindicated for mild epithelial dysplasia.

Sensitivity of direct tissue fluorescence visualization in screening for oral premalignant lesions in general practice.

Various specialty clinics and research centers have conducted studies of direct tissue fluorescence visualization as a screening technique for oral premalignant lesions and early oral squamous cell carcinoma (OSCC). The effectiveness of the VELscope in a private practice setting is unknown. This pilot study is the first report to assess the VELscope system as a screening adjunct among lower-risk populations seen by a primary care clinician in a general practice setting.

Ethical decision-making for multiple prescription dentistry.

Technology provides a selection of treatment choices for dental problems. Dental ethics must be applied to the development of a treatment plan and the selection of methods. Treatment options should consider the patient's circumstances and desires as well as the dentist's decision as it relates to best practices in dentistry.

An efficient and fully automated high-throughput transfection method for genome-scale siRNA screens.

RNA interference (RNAi), combined with the availability of genome sequences, provides an unprecedented opportunity for the massive and parallel investigations of gene function. Small interfering RNA (siRNA) represents a popular and quick approach of RNAi for in vitro loss-of-function genetic screens. Efficient transfection of siRNA is critical for unambiguous interpretation of screen results and thus overall success of any siRNA screen.