Receptors for hypothalamic releasing hormones TRH and GnRH: oligomerization and interactions with intracellular proteins.

Studies of TRH and GnRH receptors have revealed much information about the roles of G-proteins and beta-arrestins, as well as receptor residues important for signaling, desensitization and internalization. However, the proteins involved are only just beginning to be identified and characterized. Additional complexity now exists with the observation that these receptors form oligomers in live cells.

New technologies: bioluminescence resonance energy transfer (BRET) for the detection of real time interactions involving G-protein coupled receptors.

The natural phenomenon of bioluminescence resonance energy transfer (BRET) has become an extremely useful tool for studying protein-protein interactions in the laboratory, including those involving G-protein coupled receptors (GPCRs). The technology involves fusion of donor and acceptor molecules to proteins of interest. Following assessment to ensure correct functionality, co-expression of fusion constructs in live cells enables their interaction to be studied in real time in a quantitative manner.