Synthesis of a far-red emitting flavonoid-based lysosome marker for live cell imaging applications.

A bright far-red emitting flavonoid derivative (FuraET) was synthesized in good yields by inserting a π extension group (i.e., furan) into the flavonoid skeleton, via using the Suzuki-Miyaura cross-coupling reaction.

A Fluorescent Flavonoid for Lysosome Imaging: the Effect of Substituents on Selectivity and Optical Properties.

Lysosome selective bright orange-red emitting flavonoid (2) was synthesized by attaching a strong donor (NPh) group into flavonoid skeleton. As a result of efficient intra molecular charge transfer due to the strong donor group, a significant bathochromic shift was observed from the emission of 2b (with a -NPh group, λ ≈ 590 nm), in comparison that of 1b (with a -NMe group, λ ≈ 519 nm). The role of the substituent effect towards ICT was further studied by low temperature spectral analysis.

A fluorescent flavonoid for lysosome detection in live cells under "wash free" conditions.

Lysosomes are vital organelles in living cells, which have acidic environments (pH 4.0-5.0) where macrobiomolecules and malfunctioning organelles are broken down into monomers by hydrolase activity.