Probing the antioxidant activity of Δ-tetrahydrocannabinol and cannabidiol in Cannabis sativa extracts.

Herein, we report the antioxidant activity of cannabidiol (CBD) and Δ9-tetrahydrocannabinol (THC) in pure and mixed solutions at different ratios, as well as of six different Cannabis sativa extracts containing various proportions of CBD and THC by using spectrophotometric (reducing power assay, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), hypochlorous acid (HOCl) scavenging assays) and electrochemical methods (cyclic voltammetry and differential pulse voltammetry). The isolated cannabinoids, the different stoichiometric ratios of CBD and THC, and the natural extracts proved to have remarkable antioxidant properties in all the methods employed in this work. The antioxidant activity of CBD and THC was compared against that of the well-defined antioxidants such as ascorbic acid (AA), resveratrol (Resv) and (-)-epigallocatechin-3-gallate (EGCG).

A novel method for genetic transformation of using modified-hydroxyapatite nanoparticles as a plasmid DNA vehicle.

In modern biological research, genetic transformation is an important molecular biology technique with extensive applications. In this work, we describe a new method for the delivery of plasmid DNA (pDNA) into a yeast species, . This method is based on the use of novel arginine-glucose-PEG functionalized hydroxyapatite nanoparticles (M-HAp NPs) as a vehicle which delivers pDNA into with a high transformation efficiency of 10 cfu μg of pDNA, without the need for preparation of competent cells.

Novel one step transformation method for Escherichia coli and Staphylococcus aureus using arginine-glucose functionalized hydroxyapatite nanoparticles.

Bacterial gene transformation is one of the important techniques in molecular biology which has significant applications in gene cloning technology. In this study, we have developed arginine-glucose functionalized hydroxyapatite nanoparticle (R-G-HAp NPs) mediated novel one step transformation method, effective for both Gram-positive and Gram-negative bacteria. R-G-HAp NPs served as carriers to deliver pDNA into Escherichia coli and Staphylococcus aureus at room temperature, without the need for preparation of competent cells.

Self-Activated Fluorescent Hydroxyapatite Nanoparticles: A Promising Agent for Bioimaging and Biolabeling.

Bioimaging has drastically transformed the field of medicine, and made the process of diagnosis easy and fast. Visualization of complete organ to complex biological processes has now become possible. Among the various imaging processes, fluorescence imaging using nontoxic fluorescent nanomaterials is advantageous for several beneficial features including high sensitivity, minimal invasiveness, and safe detection limit.

Nanomaterial-Based Approaches for Prevention of Biofilm-Associated Infections on Medical Devices and Implants.

Biofilm formation is a major problem in medical device-related infections leading to failure of implant-based therapies. Though various conventional approaches to counter biofilm formation like physical and/or mechanical removal, chemical removal, and the use of antimicrobials exist, they fail due to increased resistance of biofilms. This review discusses various nanomaterial-based approaches such as the use of metallic and metal oxide nanoparticles- and polymer-based nanocomposites, which are currently being developed for prevention and treatment of biofilms.