Effects of Sequential Antimicrobial Phases on Root Canal Microbiome Dynamics in Two-Visit Treatment of Primary Apical Periodontitis: A Longitudinal Experimental Study.

Background: Effective management of primary apical periodontitis depends on understanding the dynamic interactions within the root canal microbiome. This study aimed to investigate the effect of sequential antimicrobial phases on the root canal microbiome during a two-visit treatment approach, with a focus on calcium hydroxide medication.

Methods: Samples were collected from three teeth across four treatment phases: initial infection (S1), after chemomechanical preparation (S2), after intracanal medication (S3), and after a final flush (S4).

Do effervescent vitamin tablets affect the surface roughness, microhardness, and color of human enamel and contemporary composite resins?

Purpose: To compare the impact of four effervescent vitamin tablets on the surface roughness, microhardness, and color of human enamel and contemporary composite resins.

Material And Methods: Sixty enamel and a total of 240 anterior (Gradia Direct Anterior), posterior (Gradia Direct Posterior), and universal composite resin (Filtek Ultimate) specimens (n = 60 per group) were fabricated. Each group was subdivided into five subgroups (n = 12, per subgroup).

Amplicon-based next-generation sequencing for comparative analysis of root canal microbiome of teeth with primary and persistent/secondary endodontic infections.

Objectives: To compare the root canal microbiome profiles of primary and persistent/secondary infections using high-throughput sequencing with the help of a reliable bioinformatics algorithm.

Materials And Methods: Root canal samples of 10 teeth in the primary endodontic infection (PEI) group and 10 teeth in the persistent/secondary endodontic infection (SEI) group were included resulting in a total of 20 samples. After DNA extraction from the samples, sequencing was performed on the Illumina MiSeq platform.

Microbiota of Endodontically Infected Primary and Permanent Teeth.

Differences in the endodontic microbiome of permanent and primary teeth during the mixed dentition period are still unknown. The purpose of this study was to examine bacterial diversity in endodontically infected primary and permanent teeth using 16S rRNA gene sequencing and the QIIME 2 (Quantitative Insights Into Microbial Ecology 2) bioinformatics pipeline. Microbial samples from endodontically infected primary ( equals 15) and permanent ( equals 15) maxillary or mandibular molar teeth were subjected to next-generation sequencing analysis based on examination of the hypervariable V3 to V4 region of the 16S rRNA gene.

Effects of attachment type and palatal coverage on oral perception and patient satisfaction in maxillary implant-supported complete denture patients.

Aim: The effect of oral rehabilitation on the oral perception of implant-supported overdenture patients is a concern. This study evaluated the effects of the attachment type and palatal coverage on oral perception and patient satisfaction in maxillary implant-supported complete denture patients.

Subjects And Methods: The correlation between oral perceptual ability (OPA) and patient satisfaction in three groups was investigated.

Effect of chitosan on sealer penetration into the dentinal tubules.

Objective: The objective of this study is to compare the effectiveness of final irrigation with chitosan, ethylenediaminetetraacetic acid (EDTA), and citric acid (CA) on a resin-based sealer (AH plus sealer [Dentsply DeTrey, Konstanz, Germany]) penetration into dentinal tubules using confocal laser scanning microscopy.

Materials And Methods: Seventy recently extracted human mandibular premolars were instrumented and irrigated with sodium hypochlorite (NaOCl), then divided into four groups according to the final irrigation regimen used: (1) the EDTA group: 17% EDTA + 2.5% NaOCl, (2) the CA group: 10% CA + 2.

Outcomes of long term treatments of type I hereditary angioedema in a Turkish family.

Background: Hereditary angioedema is a rare autosomal dominantly inherited immunodeficiency disorder characterized by potentially life-threatening angioedema attacks.

Objective: We aimed to investigate the clinical and genetic features of a family with angioedema attacks.

Methods: The medical history, clinical features and C1-INH gene mutation of a Turkish family were investigated and outcomes of long-term treatments were described.

Evaluation of dentinal defects during root canal preparation using thermomechanically processed nickel-titanium files.

Objective: The aim of this study was to compare the incidence of root cracks after root canal instrumentation with thermomechanically processed nickel-titanium (Ni-Ti) files with different instrumentation kinematics.

Materials And Methods: A total of 150 extracted mandibular premolars with mature apices and straight root canals were divided into five groups and used in this study. In Group 1, 30 teeth were prepared using hand K-files and assigned to control group, Group 2 was instrumented using K3XF Rotary files (SybronEndo, Glendora, CA, USA) with continuous rotary motion.

Efficacy of manual and mechanical instrumentation techniques for removal of overextended root canal filling material.

Objective: To compare the efficacy of manual and mechanical instrumentation techniques, including ProTaper Universal retreatment system, Mtwo retreatment system, Reciproc system, and Hedström files, regarding removal of overextended root canal filling material.

Materials And Methods: Eighty extracted human mandibular premolar teeth were prepared at the apical foramen level using Revo-S rotary files and subsequently obturated. The root canal filling material was deliberately extruded from the apex.

The effects of different nickel-titanium instruments on dentinal microcrack formations during root canal preparation.

Objective: The aim of the present study was to investigate the incidence of dentinal microcracks caused by different preparation techniques.

Materials And Methods: 120 extracted human mandibular incisor teeth were divided into five experimental groups and one control group (n = 20): Group 1: Hand preparation with balanced force technique up to #25 K-file. Group 2: Preparation with only ProTaper F2 instrument in a reciprocating movement.

Cytotoxic and genotoxic effects on gingival fibroblasts from static magnetic fields produced by dental magnetic attachments.

Objective: To investigate cytotoxic and genotoxic effects of static magnetic field (SMF) produced by dental magnetic attachments on human gingival fibroblasts in vitro.

Background: Magnetic attachments have numerous roles in dental prosthesis fixation, but few reports evaluate possible biological effects of static magnetic field (SMF) on human gingival tissues, particular genotoxic effects.

Materials And Methods: The Dyna (500-gr breakaway force) and Steco (173-gr breakaway force) dental magnetic attachments were embedded into autopolymerising acrylic resin in four different configurations each, including single and double magnets.

The effect of reciprocation versus rotational movement on the incidence of root defects during retreatment procedures.

Aim: To compare the incidence of dentinal defects caused by reciprocating and rotary techniques during retreatment procedures.

Methodology: One hundred and twenty extracted mandibular premolars with single canals were selected. Twenty teeth were left unprepared.

The effect of various backfilling techniques on the fracture resistance of simulated immature teeth performed apical plug with Biodentine.

Aim: To evaluate the fracture resistance of simulated immature teeth that had been backfilled using different materials after using Biodentine as the apical plug material.

Design: Seventy-five single-rooted teeth were divided into five groups (n = 15). The 15 teeth in group 1 served as a negative control group and received no treatment.

Effects of metal surface grinding at the porcelain try-in stage of fixed dental prostheses.

Purpose: This study was to evaluate the effect of grinding of the inner metal surface during the porcelain try-in stage on metal-porcelain bonding considering the maximum temperature and the vibration of samples.

Materials And Methods: Ninety-one square prism-shaped (1 × 1 × 1.5 mm) nickel-chrome cast frameworks 0.

Incidence of apical crack initiation and propagation during the removal of root canal filling material with ProTaper and Mtwo rotary nickel-titanium retreatment instruments and hand files.

Introduction: The aim of this study was to determine the incidence of crack initiation and propagation in apical root dentin after retreatment procedures performed by using 2 rotary retreatment systems and hand files with additional instrumentation.

Methods: Eighty extracted mandibular premolars with single canals were selected. One millimeter from the apex of each tooth was ground perpendicular to the long axis of the tooth, and the apical surface was polished.

Efficacy of different irrigation techniques in the removal of calcium hydroxide from a simulated internal root resorption cavity.

Aim: To evaluate the efficacy of six irrigation techniques to remove calcium hydroxide (CH) from a simulated internal root resorption cavity.

Methodology: The root canals of 100 single-rooted teeth were prepared using the ProTaper system to size F5. The roots were split longitudinally, and standardized simulated internal root resorption cavities were prepared in the two root halves.

Bernard Lerer: recipient of the 2014 inaugural Werner Kalow Responsible Innovation Prize in Global Omics and Personalized Medicine (Pacific Rim Association for Clinical Pharmacogenetics).

This article announces the recipient of the 2014 inaugural Werner Kalow Responsible Innovation Prize in Global Omics and Personalized Medicine by the Pacific Rim Association for Clinical Pharmacogenetics (PRACP): Bernard Lerer, professor of psychiatry and director of the Biological Psychiatry Laboratory, Hadassah-Hebrew University Medical Center, Jerusalem, Israel. The Werner Kalow Responsible Innovation Prize is given to an exceptional interdisciplinary scholar who has made highly innovative and enduring contributions to global omics science and personalized medicine, with both vertical and horizontal (transdisciplinary) impacts. The prize is established in memory of a beloved colleague, mentor, and friend, the late Professor Werner Kalow, who cultivated the idea and practice of pharmacogenetics in modern therapeutics commencing in the 1950s.

The bond strength of endodontic sealers to root dentine exposed to different gutta-percha solvents.

Aim: To evaluate the effects of various gutta-percha solvents on the push-out bond strength of several root canal sealers on root dentine.

Methodology: The root canals of 210 single-rooted human teeth were prepared with the ProTaper System (Dentsply Maillefer, Ballaigues, Switzerland) up to a master apical file size of F4, and the following variables evaluated for bond strength: solvent type (chloroform, eucalyptol and orange oil), time (2 and 5 min), sealer type (AH Plus, MTA Fillapex and Sealapex) and root thirds (coronal, middle and apical). After canal filling, three 1-mm-thick slices were obtained from each root sample, and the bond strength of the test materials was measured using a push-out test set-up at a cross-head speed of 1 mm min(-1) .

Effect of agitation of EDTA with 808-nanometer diode laser on removal of smear layer.

Introduction: Near-infrared diode lasers can be used for several applications, which range from disinfection to smear layer removal in endodontics. This study evaluated the efficacy of agitation of 15% EDTA with an 808-nm diode laser on removal of the smear layer.

Methods: Sixty extracted human maxillary central incisor teeth were instrumented up to ProTaper F4 (Dentsply Maillefer, Ballagues, Switzerland) and then randomly divided into 6 groups (n = 10 for each group) according to the different final irrigating protocols as follows: 5% sodium hypochlorite for 120 seconds performed with the NaviTip (Dentsply Maillefer, Ballaigues, Switzerland) (control group); 15% EDTA for 120 seconds performed with the NaviTip; and agitation of 15% EDTA with an 808-nm diode laser for 10, 20, 30, and 40 seconds.

Do blood contamination and haemostatic agents affect microtensile bond strength of dual cured resin cement to dentin?

Objective: The purpose of this study was to evaluate the effects of blood contamination and haemostatic agents such as Ankaferd Blood Stopper (ABS) and hydrogen peroxide (H2O2) on the microtensile bond strength between dual cured resin cement-dentin interface.

Material And Methods: Twelve pressed lithium disilicate glass ceramics were luted to flat occlusal dentin surfaces with Panavia F under the following conditions:

Control Group: no contamination, Group Blood: blood contamination, Group ABS: ABS contamination Group H2O2: H2O2 contamination. The specimens were sectioned to the beams and microtensile testing was carried out.

Evaluation of the retention force of double conical crowns used in combination with a galvanoforming and casting fabrication technique.

Purpose: The aim of this study was to measure the in vitro retention force of double conical crowns fabricated using primary galvanoforming and secondary casting techniques and those fabricated using primary casting and secondary galvanoforming techniques under simulated clinical conditions before and after a wear test.

Materials And Methods: Primary galvanoformed crowns (n = 10) with non-noble secondary crowns (n = 10; group A) and primary non-noble crowns (n = 10) with secondary galvanoformed crowns (n = 10; group B) were fabricated. Each primary and secondary crown was embedded in acrylic resin and weighed with a digital balance.

The Turkish Hereditary Angioedema Pilot Study (TURHAPS): the first Turkish series of hereditary angioedema.

Background: No published data presently exist concerning hereditary angioedema (HAE) in Turkey. The aim of the study was to initiate a preliminary multicentric evaluation about HAE and to determine the genetic properties of Turkish patients.

Methods: Based on records drawn from four medical centers we identified a total of 70 subjects, belonging to 60 unrelated families, fulfilling clinical and laboratory criteria for diagnosis of HAE with C1 inhibitor deficiency.

Treatment of patient with Papillon-Lefevre syndrome with short dental implants: a case report.

Papillon-Lefevre syndrome (PLS) is an autosomal recessive disorder. Main features of PLS are hyperkeratosis of palms and soles concomitant with premature loss of primary and permanent dentition due to progressive periodontitis. Dental management of patients with PLS is usually challenging because of early excessive loss of alveolar bone support.

Tensile bond strength of a lithium-disilicate pressed glass ceramic to dentin of different surface treatments.

The effects of desensitizer, disinfectant, saliva, blood, and hydrogen peroxide on the tensile bond strength between adhesive and ceramic as well as between adhesive and dentin were examined. Sixty 7x3 mm pressed ceramic discs of IPS e.max were fabricated and randomly assigned to six groups of different dentin surface treatments (control, desensitizer, disinfectant, saliva, blood, and hydrogen peroxide).

The effect of primers on shear bond strength of acrylic resins to different types of metals.

Statement Of Problem: Poor chemical bonding of acrylic resins to metal alloys can result in microleakage and failure of the bond. Metal primers have been shown to be effective in improving the bond strength of acrylic resins to metal alloys. However, there is insufficient information about their effects on bonding to different types of metals.