3D color doppler ultrasound for postoperative monitoring of vascularized lymph node flaps.

Background: Vascularized lymph node transfer (VLNT) is a relatively well-established microsurgical treatment for lymphedema that is especially beneficial for advanced cases in which lymphovenous anastomosis is not indicated due to lymphatic vessel sclerosis. When VLNT is performed without a skin paddle, such as a buried flap, the possibilities for postoperative monitoring are limited. The aim of our study was to evaluate the use of ultra-high-frequency color Doppler ultrasound with 3D reconstruction in a pedicled axillary lymph node flap.

Pharmacokinetics of Intramuscularly Administered Thermoresponsive Polymers.

Aqueous solutions of some polymers exhibit a lower critical solution temperature (LCST); that is, they form phase-separated aggregates when heated above a threshold temperature. Such polymers found many promising (bio)medical applications, including in situ thermogelling with controlled drug release, polymer-supported radiotherapy (brachytherapy), immunotherapy, and wound dressing, among others. Yet, despite the extensive research on medicinal applications of thermoresponsive polymers, their biodistribution and fate after administration remained unknown.

In Vivo Contrast Imaging of Rat Heart with Carbon Dioxide Foam.

Widely used classical angiography with the use of iodine contrast agents is highly problematic, particularly in patients with diabetes mellitus, cardiac and pulmonary diseases, or degree III or IV renal insufficiency. Some patients may be susceptible to allergic reaction to the iodine contrast substance. The intravenous injection of a bolus of CO (negative contrast) is an alternative method, which is, however, currently only used for imaging blood vessels of the lower limbs.

The Effect of Vascular Endothelial Growth Factor C and Adipose-Derived Stem Cells on Lymphatic Regeneration in a Rat Vascularized Lymph Node Transfer Model.

Background:  Lymphedema is a chronic condition characterized by progressive edema with complicated treatment. Recently, new treatment strategies inducing lymphangiogenesis were proposed. The aim of our study was to examine the effect of vascular endothelial growth factor C (VEGF-C) and adipose-derived stem cells (ADSCs) on lymphatic regeneration and drainage re-establishment in vascularized lymph node transfer (VLNT) model using a pedicled vascularized lymph node (VLN) groin flap.

Photoacoustic Properties of Polypyrrole Nanoparticles.

Photoacoustic imaging, an emerging modality, provides supplemental information to ultrasound imaging. We investigated the properties of polypyrrole nanoparticles, which considerably enhance contrast in photoacoustic images, in relation to the synthesis procedure and to their size. We prepared polypyrrole nanoparticles by water-based redox precipitation polymerization in the presence of ammonium persulphate (ratio Py:Oxi 1:0.

Condensed Clustered Iron Oxides for Ultrahigh Photothermal Conversion and Multimodal Imaging.

Magnetic iron oxide nanocrystals (MIONs) are established as potent theranostic nanoplatforms due to their biocompatibility and the multifunctionality of their spin-active atomic framework. Recent insights have also unveiled their attractive near-infrared photothermal properties, which are, however, limited by their low near-infrared absorbance, resulting in noncompetitive photothermal conversion efficiencies (PCEs). Herein, we report on the dramatically improved photothermal conversion of condensed clustered MIONs, reaching an ultrahigh PCE of 71% at 808 nm, surpassing the so-far MION-based photothermal agents and even benchmark near-infrared photothermal nanomaterials.

Quantitative In Vivo Monitoring of Hypoxia and Vascularization of Patient-Derived Murine Xenografts of Mantle Cell Lymphoma Using Photoacoustic and Ultrasound Imaging.

Tumor oxygenation and vascularization are important parameters that determine the aggressiveness of the tumor and its resistance to cancer therapies. We introduce dual-modality ultrasound and photoacoustic imaging (US-PAI) for the direct, non-invasive real-time in vivo evaluation of oxygenation and vascularization of patient-derived xenografts (PDXs) of B-cell mantle cell lymphomas. The different optical properties of oxyhemoglobin and deoxyhemoglobin make it possible to determine oxygen saturation (sO) in tissues using PAI.

Excitation of triplet states of hypericin in water mediated by hydrotropic cromolyn sodium salt.

Hypericin (Hyp) is a hydrophobic pigment found in plants of the genus Hypericum which exhibits low levels of solubility in water. This work shows that the solubility of Hyp can be significantly increased through the addition of cromolyn disodium salt (DSCG). Performed studies using UV-VIS absorption and fluorescence spectroscopies demonstrate that Hyp remains in a predominantly biologically photodynamic active monomeric form in the presence of DSCG at concentrations ranging from 4.

Subunit analysis of mitochondrial cytochrome c oxidase and cytochrome bc by reversed-phase high-performance liquid chromatography.

A rapid separation of the ten nuclearly-encoded subunits of mitochondrial cytochrome c oxidase, and ten out of the eleven subunits of cytochrome bc, was achieved using a short, 50 mm C-reversed-phase column. The short column decreased the elution time 4-7 fold while maintaining the same resolution quality. Elution was similar to a previously published protocol, i.

[Aseptic necrosis of proximal femur in children undergone early non-operative treatment of DDH].

Aseptic necrosis of proximal femur is the most serious complication of DDH conservative treatment. 72 dysplastic hips in 48 girls and 8 boys have been evaluated. the hips were Grafs IIc, D, III and IV types, and the treatment started between 2nd and 12th week of the newborns age.

[Axial and derotational traction before surgery for slipped femoral capital epiphysis].

18 cases of slipped femoral capital epiphysis were prepared for surgery by axial and derotational traction lasting 1 to 5 (av. 2,9) weeks. In all cases significant pain relief was achieved, partially the range of passive motion in the hip was improved.

[Long-term results of pinning for slipped capital femoral epiphysis].

Clinical and radiological analysis of 39 hips in 32 patients treated with in situ pinning for light and non-severe (up to 78 degrees) slipped capital femoral epiphysis. The population of 21 boys and 11 girls aged 10-16 (av. 13.

[Sonographic assessment of stable hip reposition in newborns and infants with congenital hip dysplasia treated conservatively].

The paper presents a sonographic assessment of dysplastic hip morphology with clinically diagnosed instability after achieving stable reposition. The analysed group comprised 42 dysplastic unstable hips. They were classified according to the Graf classification as type D, III and IV.

[The prospects of the usage of hip endoprosthesis in the fractures of femur in the elderly patients].

Seven cases of comminuted, unstable trochanteric fracture of the femur treated with total or partial hip arthroplasty are presented. The age of patients at operation ranged from 69 to 88 years (mean 81.3).

[Dynamics of dysplastic hip remodelling during the first month of a child's life in an ultrasonographic image].

Remodelling of dysplastic hip in the first four weeks of life in 51 children treated with abduction diapers has been assessed sonographically. An improvement has been found in every case. The best results have been achieved in type IIIa hips, the worst in type IV (after Graf).