Publications by authors named "Kerstin Pflieger"
The migration of cells within a three-dimensional extracellular matrix (ECM) depends sensitively on the biochemical and biophysical properties of the matrix. An example for a biological ECM is given by reconstituted basal lamina gels purified from the Engelbreth-Holm-Swarm sarcoma of mice. Here, we compare four different commercial variants of this ECM, which have all been purified according to the same protocol.
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- Rapid fibrovascularization is essential for successful integration of biomaterials, and components of the plasminogen activation system, like uPA and tPA, significantly impact this process.
- Experiments showed that mice lacking uPA, tPA, or PAI-1 had impaired blood vessel and collagen formation in implants, making it easier to remove these implants than those in normal mice.
- Coating implants with recombinant versions of uPA, tPA, or PAI-1 enhanced their vascularization, suggesting a potential new method to improve biomaterial integration.
Background: CRIP1 (cysteine-rich intestinal protein 1) has been found in several tumor types, its prognostic impact and its role in cellular processes, particularly in breast cancer, are still unclear.
Methods: To elucidate the prognostic impact of CRIP1, we analyzed tissues from 113 primary invasive ductal breast carcinomas using immunohistochemistry. For the functional characterization of CRIP1, its endogenous expression was transiently downregulated in T47D and BT474 breast cancer cells and the effects analyzed by immunoblotting, WST-1 proliferation assay and invasion assay.