Clot Contraction Drives the Translocation of Procoagulant Platelets to Thrombus Surface.

Objective- After activation at the site of vascular injury, platelets differentiate into 2 subpopulations, exhibiting either proaggregatory or procoagulant phenotype. Although the functional role of proaggregatory platelets is well established, the physiological significance of procoagulant platelets, the dynamics of their formation, and spatial distribution in thrombus remain elusive. Approach and Results- Using transmission electron microscopy and fluorescence microscopy of arterial thrombi formed in vivo after ferric chloride-induced injury of carotid artery or mechanical injury of abdominal aorta in mice, we demonstrate that procoagulant platelets are located at the periphery of the formed thrombi.

[DNA methylation in the promoter regions of the laminin family genes in normal and breast carcinoma tissues].

Extracellular glycoproteins of the laminin family are essential components of basement membranes involved in a number of biological processes, including tissue differentiation, wound healing, and tumorigenesis. We present the first comprehensive study of promoter methylation status of the genes encoding laminin chains in normal tissues (peripheral blood leucocytes, buccal epithelial cells, autopsy breast tissue samples) and in breast carcinoma samples. Based on the results of this study, we divide laminin genes into three categories.

[Effectiveness of different castration methods in breast cancer patients].

Estrogens play the most important role in breast cancer oncogenesis. There are different methods used to decrease estrogen production and serum concentration: surgery, irradiation and drug-based method. In the current study 500 patients with localized surgically resectable breast cancer (infiltrative-edematous form included) or disseminated breast cancer with indication for ovarial function inhibition.

[The immediate and late results of the surgical treatment of patients with complicated forms of pulmonary tuberculosis].

Immediate and long-term outcomes of repeated and multistage operations were analyzed in 190 patients with pulmonary tuberculosis. The aspects of work rehabilitation were studied in these patients. The efficiency of repeated and stage surgical interventions in patients both with disseminated and complicated types of tuberculosis and with pleural empyemas and in those with uncomplicated postoperative disease is 87.

[Antiradical activity of retinal lipids under illumination].

Antiradical activity (ARA) of lipids from cattle in norm and under strong illumination with and without antioxidants was studied by chemoluminiscence method of initiated ethylbenzol oxidation. Lipids ARA from the retina in norm was established to be sufficiently high-3.0 X 10(6) l/mol.

[Effect of the functional groups of tocopherol molecules on lipid viscosity of mitochondria].

The influence of tocopherol and its analogue (oxychroman) on the microviscosity of mitochondrial lipids was studied, using spin labels. The viscosity of the lipid bilayer was shown to enhance with the increase in the antioxidant content in the membrane. Small concentrations of alpha-tocopherol (10(-5)-10(-6) mol/l) were shown to increase, while large concentrations (10(-3)-10(-4) mol/l) decreased the fluidity of the lipid bilayer.

[Interrelation between the natural antioxidant content and lipid viscosity in normal organelle membranes].

Two systems regulating lipid peroxidation (LP) natural antioxidants and structural organization were studied in the liver mitochondria, microsomes and nuclear membranes, using chemiluminescent and spin labelling techniques. Mitochondrial lipids were most protected against LP and were characterized by the maximum content of effective antioxidants and high viscosity. Nuclear lipids were the least protected against the systems studied and had lower antioxidant efficiency and viscosity.

[Lipid peroxidation in external and internal mitochondrial membranes during anoxia].

Accumulation of lipid peroxidation (LPO) products was investigated in external and internal membranes of mitochondria with anoxia. The increase in LPO intensity in mitochondria membranes during hypoxia was shown to be more expressed in external membranes, with an active involvement of phospholipase A2 in the process revealed. Greater LPO intensity and lability of lysosomal membranes caused by contacts with mitochondria with anoxia have been established.