Leukaemia exposure alters the transcriptional profile and function of BCR::ABL1 negative macrophages in the bone marrow niche.

Macrophages are fundamental cells of the innate immune system that support normal haematopoiesis and play roles in both anti-cancer immunity and tumour progression. Here we use a chimeric mouse model of chronic myeloid leukaemia (CML) and human bone marrow (BM) derived macrophages to study the impact of the dysregulated BM microenvironment on bystander macrophages. Utilising single-cell RNA sequencing (scRNA-seq) of Philadelphia chromosome (Ph) negative macrophages we reveal unique subpopulations of immature macrophages residing in the CML BM microenvironment.

Spinal cord haemangiosarcoma in one dog - Case report.

A 5-year-old intact female Shih Tzu was presented with acute onset of hind leg paralysis. The neurologic examination revealed severe T3-L3 myelopathy. The differential diagnoses included degenerative, anomalous, traumatic, inflammatory, vascular, metabolic, and neoplastic changes.

Retrospective analysis of tracheal hypoplasia in brachycephalic and non-brachycephalic dogs: Inter- and intra-observer agreement of measurements.

This retrospective study was undertaken on the records of intraluminal diameter of the trachea in 185 dogs, in which hypoplasia of the trachea had been suspected. The relative size of the trachea was measured using the tracheal diameter (TD), thoracic inlet distance (TI), thoracic tracheal diameter (TT) and the width of the third rib (3R), expressed as ratios TD:TI and TT:3R. Thirty-five dogs were diagnosed as having tracheal hypoplasia.

Workers' compensation experience in some Indigenous Northern Ontario communities.

Background: There is a dearth of research about occupational health and safety experience in Indigenous communities and compensation applications from Indigenous workers appear limited.

Objective: This qualitative descriptive study was designed to explore workers' compensation experiences in some Canadian Indigenous communities.

Methods: A community-based participatory research approach was used to conduct focus groups (n = 25 participants) in three Northeastern Ontario (NEO) Indigenous communities and at one NEO Indigenous employment centre.

S2 Reactions with an Ambident Nucleophile: A Benchmark Ab Initio Study of the CN + CHY [Y = F, Cl, Br, and I] Systems.

We characterize the Walden-inversion, front-side attack, and double-inversion S2 pathways leading to Y + CHCN/CHNC and the product channels of proton abstraction (HCN/HNC + CHY), hydride-ion substitution (H + YHCCN/YHCNC), halogen abstraction (YCN/YNC + CH and YCN/YNC + CH), and YHCN/YHNC complex formation (YHCN/YHNC + CH) of the CN + CHY [Y = F, Cl, Br, and I] reactions. Benchmark structures and frequencies are computed at the CCSD(T)-F12b/aug-cc-pVTZ level of theory, and a composite approach is employed to obtain relative energies with sub-chemical accuracy considering (a) basis-set effects up to aug-cc-pVQZ, (b) post-CCSD(T) correlation up to CCSDT(Q), (c) core correlation, (d) relativistic effects, and (e) zero-point energy corrections. C-C bond formation is both thermodynamically and kinetically more preferred than N-C bond formation, though the kinetic preference is less significant.

Insights into the molecular basis of the palmitoylation and depalmitoylation of NCX1.

Catalyzed by zDHHC-PAT enzymes and reversed by thioesterases, protein palmitoylation is the only post-translational modification recognized to regulate the sodium/calcium exchanger NCX1. NCX1 palmitoylation occurs at a single site at position 739 in its large regulatory intracellular loop. An amphipathic ɑ-helix between residues 740-756 is a critical for NCX1 palmitoylation.

A Recurrent Suprapituitary Ependymal Cyst Managed by Endoscopy-Assisted Transsphenoidal Surgery in a Canine: A Case Report.

A 9-years-old spayed female mixed-breed dog was referred for the evaluation of intermittent head tremors, obtundation, long-standing blindness, and a tendency to seek confined spaces. The dog lost its vision 6 months before the current presentation. A menace response was absent on ophthalmological examination.

Systematic review: Lost-time injuries in the US mining industry.

Background: The mining industry is associated with high levels of accidents, injuries and illnesses. Lost-time injuries are useful measures of health and safety in mines, and the effectiveness of its safety programmes.

Aims: To identify the type of lost-time injuries in the US mining workforce and to examine predictors of these occupational injuries.

MEDIASTINAL LYMPHOMA AND CHYLOTHORAX IN A STRIPED SKUNK (MEPHITIS MEPHITIS).

Tumors are infrequently reported in skunks, with only a few case reports published in the literature. Chylothorax associated with mediastinal lymphoma was diagnosed in a captive 7-yr-old male striped skunk ( Mephitis mephitis ). The animal presented with anorexia and apathy.

Sensitivity and specificity of Addenbrooke's Cognitive Examination, Mattis Dementia Rating Scale, Frontal Assessment Battery and Mini Mental State Examination for diagnosing dementia in Parkinson's disease.

Introduction: Among the non-motor features of Parkinson's disease (PD), cognitive impairment is one of the most troublesome problems. Highly sensitive and specific screening instruments for detecting dementia in PD (PDD) are required in the clinical practice.

Methods: In our study we evaluated the sensitivity and specificity of different neuropsychological tests (Addenbrooke's Cognitive Examination, ACE; Frontal Assessment Battery, FAB and Mattis Dementia Rating Scale, MDRS) in 73 Parkinson's disease patients without depression.

[Sentinel lymph node examination in breast carcinoma in situ].

Introduction: Breast carcinoma in situ is defined as non-invasive form of carcinoma. However, we can see infiltration of sentinel lymph node even in this disease. The aim of this work was to determine indication criteria for detection of sentinel lymph node in breast carcinoma in situ.

Reorganization of motor system in Parkinson's disease.

Background/aims: We investigated adaptive reorganization in Parkinson's disease (PD) by fMRI using a passive movement task and compared the brain activation patterns of 10 patients with left- versus right-sided dominant symptoms. Five healthy controls were also investigated with the same settings.

Methods: We grouped patients according to the predominant side of symptoms; thus, a right-sided dominant and a left-sided dominant group was formed.

Mr imaging features of surgically induced core lesions in the equine superficial digital flexor tendon.

Tendon injuries are common in athletic humans and horses. Ultrasonography is the diagnostic method of choice in horses with tendon injuries but there is increasing application of magnetic resonance (MR) imaging to monitor and follow-up tendon healing. A core lesion was created in the superficial digital flexor tendon (SDFT) of each forelimb of four horses.

Improved identification of the palmar fibrocartilage of the navicular bone with saline magnetic resonance bursography.

Fibrocartilage degeneration is the earliest pathologic finding in navicular disease but remains difficult to detect, even with magnetic resonance (MR) imaging. We hypothesized that injection of the navicular bursa with saline would improve accuracy of MR imaging evaluation of palmar fibrocartilage. Thoracic limbs were collected from 11 horses within 6 h of death.

The role of burnout among Hungarian nurses.

This study was aimed to explore the occurrence of burnout among nurses in health and social institutions in Baranya County of Hungary, to reveal the connections between burnout and sociodemographic factors, and to learn its extent in different types of care. The survey was a one-off, representative sample with 805 questionnaires processed. The questionnaire was an internationally used and accepted standard paper designed for assessing burnout syndrome.

Acenocoumarol is not a safe alternative for anticoagulation in phenprocoumon-induced hepatic failure. Report of two cases.

Acenocoumarol has been proposed repeatedly as a safe alternative drug regimen for oral anticoagulation in patients who have suffered phenprocoumon-induced fulminant hepatic failure. The current report describes 2 patients with phenprocoumon-induced hepatic failure, necessitating liver transplantation in 1 case, who showed recurrence of liver damage when oral anticoagulation with acenocoumarol was attempted. Thus, acenocoumarol may not be a safe therapeutic alternative for patients who survived severe phenprocoumon-induced hepatotoxicity.

Sensitivity and specificity of the fine needle aspiration biopsy of the thyroid: clinical point of view.

Introduction: The rates of sensitivity and specificity of fine needle aspiration biopsy (FNAB) for the diagnosis of thyroid malignancy differ considerably among various reported series. These values are influenced by three factors: (a) whether only clearly positive and negative results are considered, or whether the commonly encountered 10-20% of indeterminate/suspicious ones are included; (b) whether adenomas are considered as neoplasms in one group with carcinomas; and (c) whether only histologically proven cases are used in calculations or whether patients with benign clinical follow-up are included.

Aim: The aim of the study was to evaluate the sensitivity and specificity of FNABs performed at this institution in the last 7 years from the clinical point of view, considering only benign vs.

Epithelial-myoepithelial carcinoma of the bronchus.

An extremely rare case of epithelial-myoepithelial carcinoma (EMC) of a lobar bronchus in a 47-year-old female is reported. Grossly, the tumor formed a polypoid mass obstructing the bronchial lumen. Microscopically, it was composed of two cellular types--epithelial cells with eosinophilic cytoplasm and clear myoepithelial cells.

[Differentiating parathyroid adenoma from microfollicular thyroid nodule in fine-needle aspiration cytology].

Two cases of fine needle aspiration cytology of parathyroid adenoma were not misinterpreted as microfollicular nodules of the thyroid. Discrimination of those two lesion is a diagnostic pitfall in cytological material. Authors pointed out criteria for differential diagnosis.

[Needle aspiration cytology of soft tissue tumours of the locomotor apparatus.].

The authors present an account of their five-year experience and results as regards the diagnosis of soft tissue tumours of the locomotor system suspect of malignity, using the method of needle aspiration biopsy. It is a semi-invasive examination with cytological evaluation of the aspirate from the tumour foci. The representativeness of the collected sample is, depending on anatomical conditions, checked by sonography.

Serum soluble IL-2 receptor as a reliable and noninvasive marker of disease activity in patients with hairy cell leukemia.

Hairy cell leukemia is a chronic lymphoproliferative disorder of B-cell lineage. Malignant cells express the interleukin-2 receptor (IL-2R) which is released in vitro as well as in vivo. The sera of patients with hairy cell leukemia contain elevated levels of this soluble receptor (sIL-2R).

Desmoplastic cellular blue nevi.

We describe five cases of cellular blue nevus (CBN) accompanied by an abundant desmoplastic reaction. The cells within the desmoplastic regions reacted strongly immunohistochemically with antibodies to S-100 protein and HMB-45 and were negative to antibodies to smooth muscle actin and desmin, providing evidence that the abundant collagen deposits were the product of the nevus cells. The microscopic picture of desmoplastic CBN can differ considerably from that of common CBN, and differential diagnosis is discussed.

The value of bone marrow biopsy in the prognosis of hairy cell leukemia (HCL).

Histological findings in the bone marrow and their changes in the course of the disease and therapy were evaluated with respect to the prognosis in a group of 32 patients with hairy cell leukemia (HCL). Two types of bone marrow infiltration by hairy cells (HCs), the diffuse type and the interstitial type, respectively were found. The diffuse type of infiltration and the minimal or absent residual hematopoiesis (RH) at presentation were found with statistical significance to be unfavorable prognostic findings when compared with interstitial infiltration and persisting RH (p < 0.

Long-term results in hairy cell leukemia treated by splenectomy.

Splenectomy as a therapeutic modality has been evaluated in a group of 24 consecutive patients. Before and after splenectomy only supportive therapy was applied with the exception of two patients in whom corticosteroids were administered because of vasculitis. Radiation therapy was applied in one patient because of massive retroperitoneal lymphadenopathy.