Characterization of highly radiosensitive cell lines from a human ovarian small-cell cancer.

Cells were obtained at paracentesis from a patient with a rapidly growing ovarian tumor. A monolayer cell line (V7S), a xenograft tumor line (V7), and subsequently a xenograft-derived monolayer cell line (V7M) were established. Histological and immunohistochemical studies of the original tumor, xenograft, and cell lines provided a diagnosis of small-cell carcinoma of the ovary-which is consistent with the clinical course of the patient.

Determination of radiation-induced damage in lymphocytes using the micronucleus and microgel electrophoresis 'Comet' assays.

DNA damage assays may be useful as rapid predictors of normal tissue radiosensitivity in clinical samples. We measured in vitro radiation-induced (2 Gy) damage to lymphocytes from cancer patients and normal healthy donors using both the micronucleus and microgel electrophoresis (Comet) assays simultaneously. For damage assessment, there was a good correlation (P < 0.

The prognostic significance of hyperploid cells in squamous carcinoma of the uterine cervix treated with radiotherapy.

Smears were obtained from patients undergoing radiotherapy for Stage I-IV squamous cell carcinoma of the cervix. The presence of cells with large or multiple nuclei in smears taken during or not more than 1 month following therapy was found to be associated with a significantly increased risk of early relapse.

A comparison of the toxicity and efficacy of cisplatin and carboplatin in advanced ovarian cancer. The Swons Gynaecological Cancer Group.

Eighty-eight patients with stage IIB-III epithelial ovarian cancer were randomised to receive first line single agent cisplatin (100 mg/m2) monthly or carboplatin (400 mg/m2) monthly for up to 5 cycles. Crossover to the opposite analogue occurred with progression or lack of response. All patients were premedicated with i.

Routine clinical application of the lymphocyte fluorescence polarization test for malignant disease.

The technique of Lymphocyte Fluorescence Polarization (LFP) can confirm or exclude the presence of malignant disease. The results from tests on a total of 566 subjects showed a false-positive rate of 3.8% and a false-negative rate of 2.

Specific absorption rate and tissue temperature in local hyperthermia.

Specific absorption rate (SAR) and tissue temperature were measured for a total of 83 treatments in 33 patients who received local hyperthermia treatment for cancer. The patients were grouped into three categories according to tumor size. Hyperthermia was induced by 13.

Serum haptoglobin and alpha 1-acid glycoprotein as indicators of the effectiveness of cis-diamminedichloroplatinum (CDDP) in ovarian cancer patients--a preliminary report.

Twenty-one patients with adenocarcinoma of the ovary were given a maximum of five courses of CDDP by i.v. infusion every 4 weeks.

Changes in serum acute phase proteins in ovarian cancer patients receiving cis-diamminedichloroplatinum (CDDP) infusion therapy.

Nine patients with adenocarcinoma of the ovary were given a number of courses of Cisplatin, by I.V. infusion.

A clinical assessment of fluorescence polarisation changes in lymphocytes stimulated by phytohaemagglutinin (PHA) in malignant and benign diseases.

The modified double-zone SCM technique, developed in these laboratories as an in vitro test for cancer, is based on the differential response to PHA of lymphocytes harvested from two regions of a Ficoll-Triosil gradient. Lymphocyte responses are measured by changes in intracellular fluorescein fluorescence polarisation. We report its continued clinical evaluation in patients hospitalised with malignant and non-malignant diseases, including a blind trial of 78 patients with disorders of the gastrointestinal tract, breast and lung.

Cancer-specific density changes in lymphocytes after stimulation with phytohaemagglutinin.

A fluorescence polarisation technique ("S.C.M.

Stromal endometriosis.

The case histories of six patients with stromal endometriosis of the uterus have been studied. These patients had been referred with disease recurrent after hysterectomy for palliative radiotherapy, which four received with useful effect. The disease has a different natural history from the commoner endometrial sarcoma, and it is suggested that combined surgery and prophylactic pelvic radiotherapy may be the best form of treatment.