Pro-inflammatory diets promote the formation of hyperuricemia.

Background: Hyperuricemia, as a very prevalent chronic metabolic disease with increasing prevalence year by year, poses a significant burden on individual patients as well as on the global health care and disease burden, and there is growing evidence that it is associated with other underlying diseases such as hypertension and cardiovascular disease. The association between hyperuricemia and dietary inflammatory index (DII) scores was investigated in this study.

Methods: This study enrolled 13, 040 adult subjects (aged ≥ 20 years) from the US National Health and Nutrition Survey from 2003 to 2018.

Knockdown of Brachyury Suppresses Breast Cancer Cell Proliferation and Migration via Targeting E2F3.

Breast cancer is one of the most frequently diagnosed cancer in women and is the major cause of most cancer-related deaths. We previously reported that Brachyury, as a sensitive and specific marker, has been verified to involve in the process of carcinogenesis and progression of breast cancer, but the mechanism by which Brachyury promotes breast cancer cells proliferation and migration still remains less clear. In this study, we identified that Brachyury was markedly increased in breast cancer compared with the adjacent tissues.

Overexpression of FGF2 delays the progression of osteonecrosis of the femoral head activating the PI3K/Akt signaling pathway.

Background: The purpose of the current study was to explore the role and underlying mechanism of FGF-2 in dexamethasone (DEX)-induced apoptosis in MC3T3-E1 cells.

Methods: GSE21727 was downloaded from the Gene Expression Omnibus (GEO) database to identify the differentially expressed genes (DEGs) by the limma/R package. MC3T3-E1 cells were exposed to DEX at different concentrations (0, 10, 10, 10, 10 and 10 mol/L), and cell viability, flow cytometry and TUNEL assay were used to detect cell proliferation and apoptosis.

Feasibility of total en-bloc spondylectomy on L5 by a posterior-only approach: An autopsy study.

Purpose: To obtain a better understanding of the structures around L5 vertebra and provide some anatomical evidence of the feasibility of total en-bloc spondylectomy (TES) on L5 in a posterior-only approach.

Methods: 12 simulated TESs on L5 by a posterior-only approach were conducted on human cadavers. The distance between the traction point of L4 nerve root and the dural sac (Da), the anterior-posterior diameter of the vertebral body,(Va), the distance between the start point of L4 nerve root and the traction point of L5 nerve root (Dh) and the height of the vertebral body (Vh) were measured.

Triptolide prevents osteoarthritis via inhibiting hsa-miR-20b.

Triptolide has been widely reported to exhibit potential therapeutic value in multiple inflammatory diseases, such as rheumatoid arthritis, systemic lupus erythematosus, and psoriasis. Although its safety and efficacy as an anti-inflammatory agent have been verified by many studies, the effect of triptolide on osteoarthritis (OA) was not clearly understood. In this study, we found that triptolide prevented OA development in a surgical destabilization of the medial meniscus (DMM) mouse model.

Intravenous glucocorticoid for pain control after spinal fusion: A meta-analysis of randomized controlled trials.

Objective: Postoperative pain was a common symptom after spinal surgery. This meta-analysis aimed to assess whether intravenous glucocorticoids has a beneficial role in reducing pain in patients following spinal fusion.

Methods: We systematically searched PubMed, Embase, the Cochrane Central Register of Controlled Trials, Web of Science, and Google databases, from inception to March 2, 2018.

Decreased miR-195 Expression Protects Rats from Spinal Cord Injury Primarily by Targeting HIF-1α.

Hypoxia-inducible factor 1-alpha (HIF-1α) protects hypoxic cells from apoptosis and necrosis under ischemic and anoxic conditions. miRNAs are important regulators in the genome. This study aims to explore whether miR-195 is involved in spinal cord injury through HIF-1α.

Restorative effect and mechanism of mecobalamin on sciatic nerve crush injury in mice.

Mecobalamin, a form of vitamin B12 containing a central metal element (cobalt), is one of the most important mediators of nervous system function. In the clinic, it is often used to accelerate recovery of peripheral nerves, but its molecular mechanism remains unclear. In the present study, we performed sciatic nerve crush injury in mice, followed by daily intraperitoneal administration of mecobalamin (65 μg/kg or 130 μg/kg) or saline (negative control).

Chondrogenesis of myoblasts in biodegradable poly-lactide-co-glycolide scaffolds.

Myoblasts are considered to be an alternative cell source for cell-based meniscal repair due to their multiple differentiation potentials. This study addresses the chondrogenic differentiation of myoblasts seeded into poly-lactide-co-glycolide (PLGA) scaffolds following implantation in a subcutaneous pocket of nude mice. Canine myoblasts isolated from a Beagle were expanded and seeded into PLGA scaffolds and cultured in cartilage-derived morphogenetic protein-2 (CDMP-2) and transforming growth factor-β1 (TGF-β1)-containing medium for 2 weeks in vitro.