Publications by authors named "Kentaro Sakata"
The voltage-dependent anion channel (VDAC) is an abundant and multifunctional outer mitochondrial membrane protein, playing key roles in neurodegeneration, apoptosis, and mitochondrial membrane biogenesis. Here, we present a protocol to produce and reconstitute high yields of detergent-solubilized VDAC, expressed as inclusion bodies in E. coli.
View Article and Find Full Text PDFThe oligosaccharide needed for protein N-glycosylation is assembled on a lipid carrier via a multistep pathway. Synthesis is initiated on the cytoplasmic face of the endoplasmic reticulum (ER) and completed on the luminal side after transbilayer translocation of a heptasaccharide lipid intermediate. More than 30 congenital disorders of glycosylation (CDGs) are associated with this pathway, including RFT1-CDG which results from defects in the membrane protein Rft1.
View Article and Find Full Text PDFThe oligosaccharide needed for protein -glycosylation is assembled on a lipid carrier via a multi-step pathway. Synthesis is initiated on the cytoplasmic face of the endoplasmic reticulum (ER) and completed on the luminal side after transbilayer translocation of a heptasaccharide lipid intermediate. More than 30 Congenital Disorders of Glycosylation (CDGs) are associated with this pathway, including RFT1-CDG which results from defects in the membrane protein Rft1.
View Article and Find Full Text PDFArticle Synopsis
- MCCs (membrane-coordinated complexes) are specialized structures in yeast that contain various proteins, including the core protein Pil1, which helps maintain their function.
- Research on mutant strains lacking Pil1 and other proteins showed that the Sur7 and Nce102 families of six-tetraspan membrane proteins play distinct roles in dealing with stress and contributing to normal assembly of MCCs.
- The study revealed that the stress sensitivity in specific mutants is linked to an overactive signaling pathway involving TOR kinase complex 2 (TORC2), and that Pil1 and 6-Tsp work together to regulate this signaling during stress responses.
Article Synopsis
- Saccharomyces cerevisiae LIP1 is a part of a team that helps make special fats called sphingolipids, which are important for cell membranes.
- Scientists created a new yeast strain that could be turned off with a drug called Dox, and they found that when LIP1 was off, the yeast had trouble growing and making sphingolipids.
- The research showed that when sphingolipid levels were low, a signaling pathway called TORC2 could still activate certain proteins, which helps control how sphingolipids are made in cells.
Background: It is important to restore horizontal and vertical stability to the acromioclavicular (AC) joint when treating dislocations of this joint. Most surgical stabilization techniques of the AC joint have primarily addressed the coracoclavicular ligament complex; however, these techniques may not satisfactorily restore horizontal stability to the AC joint.
Purpose: To evaluate the strength and bidirectional stability of 3 AC joint stabilizing techniques in a cadaveric model.
To investigate the effects of perinatal exposure to tetrabromobisphenol A (TBBPA), a brominated flame retardant, on the immune system, a respiratory syncytial virus (RSV) infection mouse model was utilized. Female mice were exposed to TBBPA mixed with the diet from 10 days after conception to weaning on postnatal day 21. Offspring mice were infected intranasally with A2 strain of RSV.
View Article and Find Full Text PDFObjective: Bile duct injury is an uncommon but potentially serious complication in cholecystectomy. A recognized treatment for minor biliary injury is internal biliary decompression by endoscopic retrograde cholangiopancreatography (ERCP) and stent insertion. The aim of this study was to assess the effectiveness of ERCP in the management of minor biliary injuries.
View Article and Find Full Text PDF