A novel arginine kinase from the shrimp Neocaridina denticulata: the fourth arginine kinase gene lineage.

Arginine kinases (AK) evolved independently at least three times: first at an early stage of phosphagen kinase evolution (typical AK), second from the cytoplasmic creatine kinase (CK) gene later in metazoan evolution (Stichopus AK) and third from the mitochondrial CK gene in the course of annelid radiation (Sabellastarte AK). Here, we present a possible fourth AK lineage. We amplified cDNA encoding three AKs (AK1, AK2 and AK3) from the shrimp Neocaridina denticulata, and determined their amino acid sequences (355-356 residues).

Cytoplasmic and mitochondrial creatine kinases from the skeletal muscle of sperm whale (Physeter macrocephalus). Molecular cloning and enzyme characterization.

We have amplified two cDNAs, coding for creatine kinases (CKs), from the skeletal muscle of sperm whale Physeter macrocephalus by PCR, and cloned these cDNAs into pMAL plasmid. These are the first CK cDNA and deduced amino acid sequences from cetaceans to be reported. One of the two amino acid sequences is a cytoplasmic, muscle-type isoform (MCK), while the other was identified as a sarcomeric, mitochondrial isoform (sMiCK) that included a mitochondrial targeting peptide.

Arginine kinase from the beetle Cissites cephalotes (Olivier). Molecular cloning, phylogenetic analysis and enzymatic properties.

Here, we report the PCR amplification and cloning of a cDNA for arginine kinase (AK) from the beetle Cissites cephalotes (Olivier). The cDNA is 1210bp and has an open reading frame of 1125bp and 5' and 3'-untranslated regions of 30 and 55bp, respectively. The open reading frame encodes a 374 amino acid protein with most of the residues considered necessary for AK function: five residues predicted to interact with the substrate arginine (S77, Y82, E239, C285 and E328), and five residues predicted to interact with the substrate ADP (R138, R140, R243, R294 and R323).

cDNA-derived amino acid sequences of myoglobins from nine species of whales and dolphins.

We determined the myoglobin (Mb) cDNA sequences of nine cetaceans, of which six are the first reports of Mb sequences: sei whale (Balaenoptera borealis), Bryde's whale (Balaenoptera edeni), pygmy sperm whale (Kogia breviceps), Stejneger's beaked whale (Mesoplodon stejnegeri), Longman's beaked whale (Indopacetus pacificus), and melon-headed whale (Peponocephala electra), and three confirm the previously determined chemical amino acid sequences: sperm whale (Physeter macrocephalus), common minke whale (Balaenoptera acutorostrata) and pantropical spotted dolphin (Stenella attenuata). We found two types of Mb in the skeletal muscle of pantropical spotted dolphin: Mb I with the same amino acid sequence as that deposited in the protein database, and Mb II, which differs at two amino acid residues compared with Mb I. Using an alignment of the amino acid or cDNA sequences of cetacean Mb, we constructed a phylogenetic tree by the NJ method.