Serum Glutamate dehydrogenase activity enables sensitive and specific diagnosis of hepatocellular injury in humans.

Serum activities of alanine- and aspartate- aminotransferases (ALT and AST) are considered the "gold standard" biomarkers of hepatocyte injury in clinical practice and drug development. However, due to expression of ALT and AST in myocytes, the diagnosis of hepatocellular injury in patients with underlying muscle diseases, including drug-induced muscle injury, is severely limited. Thus, we proposed glutamate dehydrogenase (GLDH) as a liver-specific alternative to serum ALT and AST.

Development of brake activity measurement method for heavy-duty vehicles.

Tailpipe PM (particulate matter) emissions have been reduced due to decades of tightening regulations, however non-tailpipe PM emissions are not regulated and are expected to become a significant source of traffic-related PM emissions. Previous studies have focused on emission measurement from laboratory and track tests. Their findings suggest brake wear PM emission rates are dependent on brake activity.

Effects off hydrogenated vegetable oil (HVO) and HVO/biodiesel blends on the physicochemical and toxicological properties of emissions from an off-road heavy-duty diesel engine.

In this study, the regulated emissions, gaseous toxics, and the physical, chemical, and toxicological properties of particulate matter (PM) emissions from a legacy off-road diesel engine operated on hydrogenated vegetable oil (HVO) and HVO blends with biodiesel were investigated. This is one of the very few studies currently available examining the emissions and potential health effects of HVO and its blends with biodiesel from diesel engines. Extended testing was conducted over the nonroad transient cycle (NRTC) and the 5-mode D2 ISO 8718 cycle.

Resistance Exercise Increases Gastrointestinal Symptoms, Markers of Gut Permeability, and Damage in Resistance-Trained Adults.

Purpose: This study aimed to determine the influence of acute resistance exercise (RE) and biological sex on subjective gastrointestinal (GI) symptoms, GI epithelial damage, and GI permeability in resistance-trained males and females.

Methods: Thirty resistance-trained men ( n = 15) and women ( n = 15) completed an RE bout and a nonexercise control (CON) session in a randomized counterbalanced design. The RE protocol used a load of 70% one-repetition maximum for 4 sets of 10 repetitions with a 90-s rest period length between sets and a 120-s rest period between exercises (squat, seated shoulder press, deadlift, bent-over row, and leg press).

Serum Biomarkers of Disease Activity in Longitudinal Assessment of Patients with ANCA-Associated Vasculitis.

Objective: Improved biomarkers of current disease activity and prediction of relapse are needed in antineutrophil cytoplasmic antibody-associated vasculitis (AAV). For clinical relevance, biomarkers must perform well longitudinally in patients on treatment and in patients with nonsevere flares.

Methods: Twenty-two proteins were measured in 347 serum samples from 74 patients with AAV enrolled in a clinical trial.

Creating the Components for Teaching Concepts.

An important dimension of Direct Instruction (DI) programs involves teaching conceptual behavior related to broadly applicable generalizations of a content domain. The current article outlines the necessary components for teaching a concept in any domain. The first step (1) is to conduct a concept analysis of the critical features that define the concept, as well as the features that vary from instance to instance of the concept.

Impact of Red Spinach Extract Supplementation on Bench Press Performance, Muscle Oxygenation, and Cognitive Function in Resistance-Trained Males.

The purpose of this study was to assess the impact of short-term dietary nitrate supplementation, in the form of red spinach extract (RSE), on bench press performance, muscle oxygenation, and cognitive function in resistance-trained males. Ten resistance-trained males participated in this randomized, cross-over, placebo-controlled, double-blind investigation. Each participant completed 7 days of either RSE (2 g; 180 mg NO) or a maltodextrin placebo (PL) in a counterbalanced fashion with a 14-day washout between treatments.

Real-world NOx emissions from heavy-duty diesel, natural gas, and diesel hybrid electric vehicles of different vocations on California roadways.

This study assessed the real-world nitrogen oxide (NOx) emissions from 50 heavy-duty vehicles of different vocations and engine technologies using portable emissions measurement systems (PEMS). This is one of the most comprehensive in-use emissions studies conducted to date, which played a key role in the development of CARB's (California Air Recourses Board) updated EMission FACtor (EMFAC) model, especially for natural gas vehicles. In-use emissions testing was performed on school and transit buses, refuse haulers, goods movement vehicles, and delivery vehicles while were driven over their normal operating routes in the South Coast Air Basin.

Bias, coverage, and asymptotic behaviour of random effects meta-analysis: a clinically driven simulation study.

Aims: The study of foundational features of meta-analysis is incomplete and continues to remain important. Using simulations we study bias and coverage and the asymptotic behaviour of the DerSimonian and Laird (D&L) meta-analysis with varying trial numbers and sizes, levels of risk, and extent of treatment effects.

Methods: With simulated data we model risk of untoward events in randomized controlled trials in meta-analyses.

Evaluating the Sensitivity and Specificity of Promising Circulating Biomarkers to Diagnose Liver Injury in Humans.

Early diagnosis of drug-induced liver injury (DILI) continues to be a major hurdle during drug development and postmarketing. The objective of this study was to evaluate the diagnostic performance of promising biomarkers of liver injury-glutamate dehydrogenase (GLDH), cytokeratin-18 (K18), caspase-cleaved K18 (ccK18), osteopontin (OPN), macrophage colony-stimulating factor (MCSF), MCSF receptor (MCSFR), and microRNA-122 (miR-122) in comparison to the traditional biomarker alanine aminotransferase (ALT). Biomarkers were evaluated individually and as a multivariate model in a cohort of acetaminophen overdose (n = 175) subjects and were further tested in cohorts of healthy adults (n = 135), patients with liver damage from various causes (n = 104), and patients with damage to the muscle (n = 74), kidney (n = 40), gastrointestinal tract (n = 37), and pancreas (n = 34).

Plasma Amino Acid Response to Whey Protein Ingestion Following 28 Days of Probiotic ( DE111) Supplementation in Active Men and Women.

Unlabelled: We sought to determine if 28 days of probiotic supplementation influenced the plasma amino acid (AA) response to acute whey protein feeding.

Methods: Twenty-two recreationally active men ( = 11; 24.3 ± 3.

Serum glutamate dehydrogenase activity enables early detection of liver injury in subjects with underlying muscle impairments.

Serum activities of alanine and aspartate aminotransferases (ALT and AST) are used as gold standard biomarkers for the diagnosis of hepatocellular injury. Since ALT and AST lack liver specificity, the diagnosis of the onset of hepatocellular injury in patients with underlying muscle impairments is severely limited. Thus, we evaluated the potential of glutamate dehydrogenase (GLDH) as a liver specific alternative biomarker of hepatocellular injury.

The MicroRNA-based Liquid Biopsy Improves Early Assessment of Lethal Acetaminophen Poisoning: A Case Report.

BACKGROUND Acetaminophen overdose is the most common cause of acute liver failure. Nevertheless, new biomarker approaches enabling early prediction of the outcome of the acetaminophen overdose are needed. Recently, using next-generation sequencing analysis of serum from human study participants we uncovered injury-specific signatures of circulating microRNAs (miRNAs) that represented underlying molecular mechanisms of toxicity.

Maximal Strength, Muscle Activation, and Bar Velocity Comparisons Between Squatting With a Traditional or Safety Squat Bar.

Vantrease, WC, Townsend, JR, Sapp, PA, Henry, RN, and Johnson, KD. Maximal strength, muscle activation, and bar velocity comparisons between squatting with a traditional or safety squat bar. J Strength Cond Res 35(2S): S1-S5, 2021-The purpose of this study was to compare strength, muscle activation, and bar velocity between the traditional (TRAD) and safety squat bar (SSB) back squat.

On-road gaseous and particulate emissions from GDI vehicles with and without gasoline particulate filters (GPFs) using portable emissions measurement systems (PEMS).

This study assessed the on-road gaseous and particulate emissions from three current technology gasoline direct injection (GDI) vehicles using portable emissions measurement systems (PEMS). Two vehicles were also retrofitted with catalyzed gasoline particulate filters (GPFs). All vehicles were exercised over four routes with different topological and environmental characteristics, representing urban, rural, highway, and high-altitude driving conditions.

Evaluation of Potential Serum Biomarkers of Disease Activity in Diverse Forms of Vasculitis.

Objective: We evaluated potential circulating biomarkers of disease activity in giant cell arteritis (GCA), Takayasu arteritis (TA), polyarteritis nodosa (PAN), and eosinophilic granulomatosis with polyangiitis (EGPA).

Methods: A panel of 22 serum proteins was tested in patients enrolled in the Vasculitis Clinical Research Consortium Longitudinal Studies of GCA, TA, PAN, or EGPA. Mixed models were used for most analyses.

The association of serum interleukin-6 levels with clinical outcomes in antineutrophil cytoplasmic antibody-associated vasculitis.

Objective: To investigate serum IL-6 (sIL-6) levels during active disease, complete remission (CR), and relapse in antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV), and to explore the association of changes in sIL-6 with clinical outcomes.

Methods: sIL-6 levels were measured at baseline and longitudinally over 18 months, in 78 patients with AAV enrolled in a randomized controlled trial comparing treatment with either rituximab (RTX) or cyclophosphamide (CYC)/azathioprine (AZA). Outcome variables included baseline clinical features, ANCA specificity, disease activity (active disease versus CR), time to relapse events, B cell repopulation, and ANCA titer increases.

On-Board Sensor-Based NO Emissions from Heavy-Duty Diesel Vehicles.

Real-world nitrogen oxides (NO ) emissions were estimated using on-board sensor readings from 72 heavy-duty diesel vehicles (HDDVs) equipped with a Selective Catalytic Reduction (SCR) system in California. The results showed that there were large differences between in-use and certification NO emissions, with 12 HDDVs emitting more than three times the standard during hot-running and idling operations in the real world. The overall NO conversion efficiencies of the SCR system on many vehicles were well below the 90% threshold that is expected for an efficient SCR system, even when the SCR system was above the optimum operating temperature threshold of 250 °C.

The Effects of Whey vs. Pea Protein on Physical Adaptations Following 8-Weeks of High-Intensity Functional Training (HIFT): A Pilot Study.

This study examined the effects of whey and pea protein supplementation on physiological adaptations following 8-weeks of high-intensity functional training (HIFT). Fifteen HIFT men (n = 8; 38.6 ± 12.

Effects of Probiotic ( DE111) Supplementation on Immune Function, Hormonal Status, and Physical Performance in Division I Baseball Players.

We sought to determine the effects of probiotic supplementation ( DE111; 1 billion CFU∙d) on markers of immune and hormonal status in collegiate male athletes following 12 weeks of offseason training. Twenty-five Division I male baseball athletes (20.1 ± 1.

Protocol of the randomised placebo controlled pilot trial of the management of acute sciatica (SCIATICA): a feasibility study.

Introduction: Acute sciatica (symptom duration less than 4 weeks), a major cause of pain and disability, is a common presentation to medical practices and hospital emergency departments. Selective CT fluoroscopy transforaminal epidural steroid injection is often used with the hope of reducing pain and improving function. Recently, there has been interest in using systemic corticosteroids in acute sciatica.

Real-world exhaust temperature and engine load distributions of on-road heavy-duty diesel vehicles in various vocations.

Real-world vehicle and engine activity data were collected from 90 heavy-duty vehicles in California, United States, most of which have engine model year 2010 or newer and are equipped with selective catalytic reduction (SCR). The 90 vehicles represent 19 different groups defined by a combination of vocational use and geographic region. The data were collected using advanced data loggers that recorded vehicle speed, position (latitude and longitude), and more than 170 engine and aftertreatment parameters (including engine load and exhaust temperature) at the frequency of one Hz.