Characterization of the physiological spaces and distribution of tolbutamide in the perfused rat pancreas.

Purpose: To set up and validate a viable perfused rat pancreas model suitable for pharmacokinetic studies.

Materials And Methods: We setup and conducted multiple indicator dilution studies in the single pass perfused rat pancreas. The distribution of the reference markers [99mTc]-red blood cells (RBC), [14C]-sucrose, and [3H]-water, and tolbutamide were analysed using both non-parametric and parametric methods.