The effect of perfusion with UW solution on the skeletal muscle and vascular endothelial exocrine function in rat hindlimbs.

Background: The effect of University of Wisconsin (UW) solution perfusion for extremity preservation is still unknown although it is widely used. The purpose of this study is to examine the effect of UW solution perfusion on skeletal muscle preservation in a rat model.

Materials And Methods: Rat hindlimbs were amputated and either preserved with UW solution perfusion (UW perfusion group) or given no perfusion (no-perfusion group) for 5 h at 25 degrees C.

Effect of nitric oxide on the contractile function of rat reperfused skeletal muscle.

Background: The involvement of nitric oxide (NO) in ischemia-reperfusion injury remains controversial and has been reported to be both beneficial and deleterious. The purpose of this study was to examine the contribution of NO and superoxide to skeletal muscle function using an ischemic revascularized hind limb model in rats.

Patients And Materials: Warm ischemia produced by vascular pedicle clamping was sustained for 3 h.