Laboratory maintenance of rodent malaria parasites.

We provide a series of protocols that have been used for the cyclic transmission of rodent malaria parasites in the laboratory. This is now possible both in vivo and in vitro. We focus on the least "resource intensive" and generic methods that we find applicable to any parasite-host combination.

Comparison of dose decrement from intrafraction motion for prone and supine prostate radiotherapy.

Background And Purpose: Dose effects of intrafraction motion during prone prostate radiotherapy are unknown. We compared prone and supine treatment using real-time tracking data to model dose coverage.

Material And Methods: Electromagnetic tracking data were analyzed for 10 patients treated prone, and 15 treated supine, with IMRT for localized prostate cancer.

Small-molecule diffusion through polycrystalline triglyceride networks quantified using fluorescence recovery after photobleaching.

Fluorescence recovery after photobleaching (FRAP) has been used to quantify Nile red diffusion through five different triglyceride crystal networks composed of pure peanut oil (PeO), pure chemically interesterified fully hydrogenated palm oil (IHPO), two blends of PeO and IHPO blended in different mass ratios (70:30 and 30:70%, w/w), and pure cocoa butter. Calculated components from FRAP experiments (effective diffusion coefficient (D(eff)) and mobile fraction (Mr)) were correlated with crystal network structural characteristics (crystalline mass fraction and permeability coefficient) and illustrated that D(eff) can be predicted using this tool. Higher-permeability coefficients found for higher fractal dimensions, lower volume fraction of solids, and larger average particle sizes were significantly correlated to higher D(eff).