Traumatic wound dehiscence after deep anterior lamellar keratoplasty.

We report the case of a 12-year-old boy with severe keratoconus who had uneventful deep anterior lamellar keratoplasty (DALK) using the big-bubble technique in the left eye for severe loss of best spectacle-corrected visual acuity and contact lens intolerance. Two weeks after surgery, the patient experienced accidental blunt trauma to the operative eye and developed a traumatic 180-degree superior wound dehiscence of the lamellar graft without perforation into the anterior chamber. The DALK technique provided adequate tectonic support to prevent a ruptured globe throughout the traumatic event in contrast to penetrating keratoplasty, which can lead to devastating vision damage after blunt trauma.

Identification of unknown intraocular material after cataract surgery: evaluation of a potential cause of toxic anterior segment syndrome.

Purpose: To describe and identify unknown opaque material between the optic of an AR40 intraocular lens (IOL) injected with the Emerald Series implantation system (both AMO, Inc.) and the posterior capsule at the conclusion of routine phacoemulsification to prevent an outbreak of toxic anterior segment syndrome (TASS).

Setting: Ambulatory care center operating room, University of North Carolina Hospitals and Department of Ophthalmology, University of North Carolina School of Medicine at Chapel Hill, Chapel Hill, North Carolina, USA.

Determining factors for corneal endothelial cell loss by using bimanual microincision phacoemulsification and power modulation.

Purpose: To determine risk factors for central corneal endothelial cell loss in bimanual microincision cataract surgery by using power modulation.

Methods: Prospective study: 79 eyes (70 subjects) with uncomplicated bimanual cataract surgery by using power modulation. Cataracts were graded with the LOCS III system.

Alterations in central neuropeptide expression, release, and receptor binding in rats bred for high anxiety: critical role of vasopressin.

To model aspects of trait anxiety/depression, Wistar rats were bred for extremes in either hyper (HAB)- or hypo(LAB)-anxiety as measured on the elevated plus-maze and in a variety of additional behavioral tests. Similar to psychiatric patients, HAB rats prefer passive stress-coping strategies, indicative of depression-like behavior, show hyper-reactivity of the hypothalamo-pituitary-adrenal axis, and a pathological response to the dexamethasone/corticotropin-releasing hormone (CRH) challenge test. Here we tested central mRNA expression, release patterns, and receptor binding of neuropeptides critically involved in the regulation of both anxiety-related behavior and the HPA axis.

Semicarbazide-sensitive amine oxidase in aortic smooth muscle cells mediates synthesis of a methylglyoxal-AGE: implications for vascular complications in diabetes.

Semicarbazide-sensitive amine oxidase (SSAO) catalyzes formation of methylglyoxal (MG) from aminoacetone; MG then reacts with proteins to form advanced glycation end products or AGEs. Because of its potential to generate MG, SSAO may contribute to AGE-associated vascular complications of aging and diabetes. We developed a method to measure SSAO activity in bovine aortic smooth muscle cells (BASMC) based on the oxidation of 2',7'-dichlorofluorescin by hydrogen peroxide and horseradish peroxidase.