The evaluation of a novel digital immunochromatographic assay with silver amplification to detect SARS-CoV-2.

Introduction: Rapid antigen tests are convenient for diagnosing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); however, they have lower sensitivities than nucleic acid amplification tests. In this study, we evaluated the diagnostic performance of Quick Chaser Auto SARS-CoV-2, a novel digital immunochromatographic assay that is expected to have higher sensitivity than conventional antigen tests.

Methods: A prospective observational study was conducted between February 8 and March 24, 2021.

Dissemination in Japan of multidrug-resistant Pseudomonas aeruginosa isolates producing IMP-type metallo-β-lactamases and AAC(6')-Iae/AAC(6')-Ib.

The spread throughout Japan of antibiotic-resistance factors in multidrug-resistant (MDR) Pseudomonas aeruginosa isolates was investigated epidemiologically, using immunochromatographic assays specific for IMP-type metallo-β-lactamases (IMPs) and aminoglycoside 6'-N-acetyltransferase [AAC(6')]-Iae and -Ib. Three hundred MDR P. aeruginosa isolates were obtained during each of two years, 2011 and 2012, from 190 hospitals in 39 prefectures in Japan.

Production of monoclonal antibodies specific for the recombinant viral coat protein of Apple stem grooving virus-citrus isolate and their application for a simple, rapid diagnosis by an immunochromatographic assay.

A simple and rapid immunochromatographic assay (ICA) for the diagnosis of Apple stem grooving virus (ASGV) in citrus was developed. Nine lines of monoclonal antibodies (mAbs) were produced by immunizing with a recombinant viral coat protein of ASGV as the antigen. According to the competitive-binding ELISA results, the 9 mAbs comprised 2 paratope groups, A and B.

Development of an immunochromatographic assay for rapid detection of AAC(6')-Ib-producing Pseudomonas aeruginosa.

To detect aminoglycoside 6'-N-acetyltransferase-Ib [AAC(6')-Ib]-producing, Pseudomonas aeruginosa isolates which are a frequent cause of nosocomial infections in Japan, an immunochromatographic assay was developed using two kinds of monoclonal antibodies (mAbs) recognizing AAC(6')-Ib. The results of the assessment were fully consistent with those of aac(6')-Ib PCR analyses.

Discrimination of influenza A subtype by antibodies recognizing host-specific amino acids in the viral nucleoprotein.

Background: Nucleoprotein (NP) of influenza viruses is utilized to differentiate between the A, B, and C viral serotypes. The availability of influenza genome sequence data has allowed us to identify specific amino acids at particular positions in viral proteins, including NP, known as "signature residues," which can be used to discriminate human influenza A viruses from H5N1 highly pathogenic avian influenza in human cases (HPAI) and pandemic H1N1(2009) (H1N1/2009) viruses.

Methods: Screening and epitope mapping of monoclonal antibodies (mAb) against NP of influenza A, which reacted differently with NP from human influenza A virus from HPAI and H1N1/2009 A virus.

Development of an immunochromatographic assay for diagnosing the production of IMP-type metallo-β-lactamases that mediate carbapenem resistance in Pseudomonas.

Rapid and reliable detection of carbapenem-resistant bacteria is an important infection-control measure and a crucial aspect of antimicrobial chemotherapy. IMP-type metallo-β-lactamase (MBL) is an emzyme that mediate carbapenem resistance in bacteria. Here, an immunochromatographic assay was newly developed using novel monoclonal antibodies (mAbs) recognizing IMP-type MBL.

Multicenter prospective evaluation of a novel rapid immunochromatographic diagnostic kit specifically detecting influenza A H1N1 2009 virus.

Background: Definitive diagnosis is crucial in reducing morbidity and mortality from pandemic influenza A H1N1 2009 (A/H1N1/2009), especially in high-risk populations. We recently developed a rapid diagnosis kit (RDK) capable of specifically detecting A/H1N1/2009.

Objectives: To evaluate the diagnostic capability of the RDK in a multicenter, prospective trial.

Development of an immunochromatographic assay for the rapid detection of AAC(6')-Iae-producing multidrug-resistant Pseudomonas aeruginosa.

Objectives: To develop an easy-to-use method for the rapid detection of antibiotic-resistant bacteria. Here, a new immunochromatographic assay specific for aminoglycoside 6'-N-acetyltransferase AAC(6')-Iae was designed. AAC(6')-Iae is a significant marker molecule for multidrug-resistant (MDR) Pseudomonas aeruginosa isolates in Japan.

Development of an immunochromatographic assay specifically detecting pandemic H1N1 (2009) influenza virus.

The pandemic caused by a new type of influenza virus, pandemic H1N1 (2009) influenza virus A (AH1pdm), has had a major worldwide impact. Since hemagglutinin (HA) genes are among the most specific genes in the influenza virus genome, AH1pdm can be definitively diagnosed by viral gene analysis targeting the HA genes. This type of analysis, however, cannot be easily performed in clinical settings.

Polar coordinate representation of Hb(rc) versus (h2/8m)nabla2rhob(rc) at BCP in AIM analysis: classification and evaluation of weak to strong interactions.

Polar coordinate (R, theta) representation is proposed for the plot of Hb(rc) versus (h2/8m)nabla2rhob(rc) in AIM analysis to classify, evaluate, and understand weak to strong interactions in a unified way and in more detail; Hb(rc) and nabla2rhob(rc) are total electron energy densities and the Laplacian of rhob(rc) at bond critical points (BCPs: rc), respectively, where rhob(rc) are electron densities at rc. Both the x- and y-axes of the plot are expressed in the common unit of energy since Hb(rc) = Gb(rc) + Vb(rc) and (h2/8m)nabla2rhob(rc) = Hb(rc) - Vb(rc)/2 (= Gb(rc) + Vb(rc)/2), where Gb(rc) and Vb(rc) are kinetic energy densities and potential energy densities, respectively. Data employed for the plot are calculated at BCPs for full-optimized structures and optimized structures with the fixed distances (r) of r = r(o) + wa(o), where r(o) are the full-optimized distances, a(o) is the Bohr radius, and w = +/-0.

Atoms-in-molecules dual parameter analysis of weak to strong interactions: behaviors of electronic energy densities versus Laplacian of electron densities at bond critical points.

AIM dual parameter analysis is proposed for the better understanding of weak to strong interactions: Total electron energy densities (H(b)(r(c))) are plotted versus Laplacian of electron densities (Delta rho(b)(r(c))) at bond critical points (BCPs). Interactions examined in this work are those in van der Waals adducts, hydrogen bonded complexes, molecular complexes and hypervalent adducts through charge transfer (CT) interactions, and some classical covalent bonds. Data calculated at BCPs for the optimized distances (r(o)), together with r(o) - 0.

Contributions from atomic p(Se), d(Se), and f(Se) orbitals to absolute paramagnetic shielding tensors in neutral and charged SeHn and some oxides including the effect of methyl and halogen substitutions on sigmap(Se).

Contributions from atomic p(Se), d(Se), and f(Se) orbitals to sigmap(Se) are evaluated for neutral and charged Se*Hn (*=null, +, or -) and some oxides to build the image of the contributions. The effect of methyl and halogen substitutions is also examined employing RrSe*XxOo (*=null, +, or -) where R=H or Me; X=F, Cl, or Br. The p(Se) contributions are larger than 96 % for SeH- (Cinfinityv), SeH2 (C2v), SeH3 + (C3v), SeH3 + (D3h), and SeH4 (Td).

Evaluation of electron population terms for 4p, 3p, and (2p): how do HOMO and LUMO shrink or expand depending on nuclear charges?

Electron population terms are evaluated for N=Se, S, and O. Calculations are performed on HOMO and LUMO constructed by pure atomic 4p(Se), 3p(S), and 2p(O) orbitals, employing the 6-311+G(3d) and/or 6-311(++)G(3df,3pd) basis sets at the HF, MP2, and DFT (B3 LYP) levels. Se(4+), Se(2+), Se(0), and Se(2-) with the O(h) symmetry are called G(A: Se) and HSe(+), H(2)Se, and HSe(-) with the C(infinityh) or C(2v) symmetry are named G(B: Se), here [G(A+B: Se) in all].

Immunochromatographic assay for simple and rapid detection of and related viruses using monoclonal antibodies.

A simple and rapid immunochromatographic assay (ICA) to detect (SDV) was developed using colloidal gold conjugates of anti-SDV monoclonal antibodies. Of six homogenization buffers tested, 0.1 M citrate buffer (pH 7.