[Comparison between biotype of Vibrio cholerae O1 and genotype using polymerase chain reaction].

To compare between biotype of Vibrio cholerae O1 and genotype using polymerase chain reaction (PCR), 9 classical and 81 El Tor biovar strains were investigated for hemolysis, agglutination of avian erythrocytes, VP test reactivity, sensitivity to both polymyxin B and classical phage IV, and genotype using PCR amplification of hlyA, tcpA, rtxA and rtxC. One classical biovar strain showed atypical reaction upon agglutination of avian erythrocytes. Eighteen El Tor biovar strains showed atypical reactions, with the exception of sensitivity to polymyxin B.

[A nosocomial outbreak of diarrhea caused by toxin A-negative, toxin B-positive Clostridium difficile in a cancer center hospital].

Between February and July 2001, 15 patients were diagnosed as Clostridium difficile-associated diarrhea in a ward of hematological neoplasm and lung cancer in a cancer center hospital. Of these 15 patients, 10 had malignant lymphoma, and 12 and 11 had exposure to antimicrobial agents and cancer chemotherapy, respectively, before the onset of diarrhea. Toxin A-positive, toxin B-positive (A+ B+) C.