Microdevice based on centrifugal effect and bifurcation law for separation of plasma from on-line diluted whole blood.

In recent decades, scientific interest in the development of devices capable of performing routine clinical analyses through the application of standardized traditional laboratory protocols in a miniaturized lab-on-a-chip device has increased. In the present work, an innovative microdevice for the on-line whole blood dilution with a phosphate buffer solution (PBS) and separation of plasma was designed, manufactured, and characterized. The microdevice was constructed with a rectangular cross-section and spiral-shaped microchannels by photolithography and soft litography.

Microdevice immunoassay with conjugated magnetic nanoparticles for rapid anti-cyclic citrullinated peptide (anti-CCP) detection.

Anti-cyclic citrullinated peptide IgG antibodies (anti-CCP) are produced as an immune response in the presence of post-translational modified peptides known as cyclic citrullinated peptides (CCP). Anti-CCP have been considered as specific biomarkers for the diagnosis of rheumatoid arthritis (RA), and due to their high specificity, it is possible to make a differential diagnosis of other rheumatic diseases. These autoantibodies can be detected in the early stages of RA and even up to 10 years before presenting the first symptoms of the disease opening a window of opportunity for timely treatment.

Rapid, Sensitive, and Selective Detection of H5 Hemagglutinin from Avian Influenza Virus Using an Immunowall Device.

Avian influenza virus (AIV) infection, caused by influenza virus type A, is an infectious, acute respiratory disease of birds related to influenza outbreaks worldwide. The highly pathogenic AIV subtype H5N1 has crossed species barriers to infect mammals, including humans, with fatal outcomes and has received attention as a potential pandemic threat. A rapid and timely detection in poultry is vitally important to prevent the virus spread.