Development of a Novel Human Serum Albumin-Based Tool for Effective Drug Discovery: The Investigation of Protein Quality and Immobilization.

The binding ability of human serum albumin (HSA) on active pharmaceutical ingredients (APIs) is one of the most important parameters in the early stages of drug discovery. In this study, an immobilized HSA-based tool was developed for the rapid and easy in vitro screening of API binding. The work explored the serious incompleteness in the identification of HSA used for in vitro screening published in the last five years.

Immobilization of human tyrosine hydroxylase onto magnetic nanoparticles - A novel formulation of a therapeutic enzyme.

Human tyrosine hydroxylase (hTH) has key role in the production of catecholamine neurotransmitters. The structure, function and regulation of hTH has been extensively researched area and the possibility of enzyme replacement therapy (ERT) involving hTH through nanocarriers has been raised as well. However, our understanding on how hTH may interact with nanocarriers is still lacking.

Effect of Binding Linkers on the Efficiency and Metabolite Profile of Biomimetic Reactions Catalyzed by Immobilized Metalloporphyrin.

The investigation of liver-related metabolic stability of a drug candidate is a widely used key strategy in early-stage drug discovery. Metalloporphyrin-based biomimetic catalysts are good and well-described models of the function of CyP450 in hepatocytes. In this research, the immobilization of an iron porphyrin was performed on nanoporous silica particles via ionic interactions.

Antibody dependent cellular cytotoxicity of lymphocytes in patients with renal diseases of immunopathological origin.

Antibody dependent cytotoxicity (ADCC) of lymphocytes from twenty-two patients with histologically proven chronic glomerulonephritis and eight patients with SLE nephropathy was studied in a xenogeneic assay on chicken red blood cells and in an allogeneic test system using Rh(+) human erythrocyte targets. ADCC activity of the lymphocytes of patients with chronic glomerulonephritis did not differ from that of normals, while all patients with SLE nephropathy showed significantly lower cytotoxicity than healthy controls. The possible role ADCC in the pathogenesis of renal diseases of immunopathological origin is discussed.

Effect of combined immunosuppressive-cytostatic treatment on antibody-dependent cellular cytotoxicity of patients with chronic glomerulonephritis.

Antibody-dependent cellular cytotoxicity by peripheral blood mononuclear cells was evaluated in 25 patients with chronic glomerulonephritis before and after combined vinblastine-cyclophosphamide-6-mercaptopurine-prednisolone therapy. Purified peripheral blood mononuclear cells of the patients mediated normal killed activity before the treatment and a strong suppression of their cytotoxic capacity was observed after the therapy. The possible significance of the inhibitory effect of immunosuppressive cytostatic treatment on human antibody-dependent cellular cytotoxicity is discussed.

The incidence of immunoglobulin-A deposits in glomerular diseases and its morphological examination: nephropathy with mesangial IgA dense deposits.

The presence of IgA with IgG, C', IgM and fibrin in 365 renal biopsy specimens has been studied by the immunohistological method. Glomerular IgA deposits were found in 38 cases (10.4%).