Publications by authors named "Kenan Ye"
Renal glomeruli have traditionally been studied by micrometer-scale optical microscopy to interrogate overall physiology or molecular distributions and by nanoscale electron microscopy to interrogate the ultrastructure of thin sections. While these approaches are powerful, they have been limited in their ability to obtain detailed views of the glomeruli as holistic 3D functional units. To fill this knowledge gap, we have developed a novel pipeline for imaging, reconstructing, and analyzing whole mouse glomeruli at 100 nm resolution using super-resolution fluorescence microscopy.
View Article and Find Full Text PDFSARS-CoV-2, the culprit pathogen of COVID-19, elicits prominent immune responses and cytokine storms. Intracellular Cl is a crucial regulator of host defense, whereas the role of Cl signaling pathway in modulating pulmonary inflammation associated with SARS-CoV-2 infection remains unclear. By using human respiratory epithelial cell lines, primary cultured human airway epithelial cells, and murine models of viral structural protein stimulation and SARS-CoV-2 direct challenge, we demonstrated that SARS-CoV-2 nucleocapsid (N) protein could interact with Smad3, which downregulated cystic fibrosis transmembrane conductance regulator (CFTR) expression via microRNA-145.
View Article and Find Full Text PDFArticle Synopsis
- - This study investigates the role of intracellular chloride (Cl) in airway epithelial cells and how its accumulation affects airway inflammation, especially after exposure to Pseudomonas aeruginosa lipopolysaccharide (LPS).
- - Researchers found that higher levels of intracellular Cl activated certain signaling pathways (NF-κB and SGK1), leading to increased airway inflammation, while inhibiting SGK1 reduced this inflammation both in lab settings and in animal models.
- - The study highlights the significance of the Cl-SGK1 signaling pathway in conditions like bronchiectasis, and suggests that targeting excessive intracellular Cl levels could be a potential strategy for treating airway inflammatory diseases.
Background: The epithelium lining the epididymis provides an optimal acidic fluid microenvironment in the epididymal tract that enable spermatozoa to complete the maturation process. The present study aims to investigate the functional role of Na(+)/HCO(3)(-) cotransporter in the pH regulation in rat epididymis.
Method/principal Findings: Immunofluorescence staining of pan cytokeratin in the primary culture of rat caput epididymal epithelium showed that the system was a suitable model for investigating the function of epididymal epithelium.
Article Synopsis
- Thrombopoietin (TPO) is crucial for platelet production and megakaryocyte activity, and interleukin 6 (IL-6) enhances TPO's effectiveness in creating megakaryocyte colonies.
- Researchers developed a high-expression system that produces a novel 78-kDa fusion protein (ZH646) combining IL-6 and TPO, achieving significant yields from recombinant yeast.
- ZH646 has been shown to significantly boost megakaryocyte formation and platelet recovery in mice, outperforming the effects of TPO or IL-6 alone, indicating its potential as a powerful therapeutic agent for improving platelet production.