Colorimetric detection of HO with FeO@Chi nanozyme modified µPADs using artificial intelligence.

Peroxidase mimicking FeO@Chitosan (FeO@Chi) nanozyme was synthesized and used for high-sensitive enzyme-free colorimetric detection of HO. The nanozyme was characterized in comparison with  FeO nanoparticles (NPs) using X-ray diffraction, Fourier-transform infrared spectroscopy, dynamic light scattering, and thermogravimetric analysis. The catalytic performance of FeO@Chi nanozyme was first evaluated by UV-Vis spectroscopy using 3,3',5,5'-tetramethylbenzidine.