Publications by authors named "Ken-ichi Inaishi"
J Biomol Struct Dyn
April 2008
Article Synopsis
- The study examines single-molecule DNA digestion using exonuclease III, which works in a 3' to 5' direction, in a micro-channel with two layers of laminar flow.
- A DNA-bead complex was trapped and stretched in the absence of exonuclease III, then moved to a flow layer containing the enzyme, initiating the digestion process.
- The reaction revealed that the DNA shortened linearly over time, and the single-molecule digestion rate was about 28 times faster than what was observed in a bulk experiment.
