Effects of A23187 and CaCl(2) on tri-n-butyltin-induced cell death in rat thymocytes.

As tri-n-butyltin (TBT), one of the environmental pollutants, is accumulated in wild animals, concern regarding the toxicity of TBT in both wildlife and human is increasing. TBT has been reported to increase intracellular Ca(2+) concentration in several types of cells. In order to examine how Ca(2+) is involved in TBT-induced cell death, the effect of TBT on rat thymocytes has been compared with that of A23187, a calcium ionophore, under various concentrations of external Ca(2+) using a flow cytometer and fluorescent probes.

Cytotoxic effects of triphenylbismuth on rat thymocytes: comparisons with bismuth chloride and triphenyltin chloride.

The biomedical and industrial uses of organobismuth compounds have become widespread, although there is limited information concerning their cytotoxicity. Therefore, the actions of triphenylbismuth on rat thymocytes were examined using a flow cytometer with ethidium bromide, annexin V-FITC, fluo-3-AM, and 5-chloromethylfluorescein (5CMF) diacetate. Triphenylbismuth at 3-30 microM increased the population of cells stained with ethidium, indicating a decrease in cell viability.