Publications by authors named "Kelvin Frank Cho"
Article Synopsis
- The study investigates the role of Lassa virus (LASV) polymerase in its life cycle, focusing on how it interacts with cellular proteins during viral RNA synthesis in infected cells.
- Researchers used a proximity proteomics technique to identify 42 key proteins that interact with the LASV polymerase and explored their potential roles in genuine LASV infections.
- They found that one specific protein, eRF3a/GSPT1, is essential for LASV replication, and targeting it with a drug candidate significantly inhibited the virus, highlighting the potential for new antiviral strategies.
Ebola virus (EBOV) critically depends on the viral polymerase to replicate and transcribe the viral RNA genome in the cytoplasm of host cells, where cellular factors can antagonize or facilitate the virus life cycle. Here we leverage proximity proteomics and conduct a small interfering RNA (siRNA) screen to define the functional interactome of EBOV polymerase. As a proof of principle, we validate two cellular mRNA decay factors from 35 identified host factors: eukaryotic peptide chain release factor subunit 3a (eRF3a/GSPT1) and up-frameshift protein 1 (UPF1).
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