Transgenic cytoplasmic incompatibility persists across age and temperature variation in .

Environmental stressors can impact the basic biology and applications of host-microbe symbioses. For example, symbiont densities and cytoplasmic incompatibility (CI) levels can decline in response to extreme temperatures and host aging. To investigate whether transgenic expression of CI-causing genes overcomes the environmental sensitivity of CI, we exposed transgenic male flies to low and high temperatures as well as aging treatments.