Publications by authors named "Kelly Jara"
Robust patient-derived platforms that recapitulate the cellular and molecular fingerprints of glioblastoma are crucial for developing effective therapies. Here, we describe a chemically defined protocol for 3D culture and propagation of glioblastoma in 3D gliospheres, patient-derived organoids (PDOs), mouse brain orthotopic xenografts (PDOXs), and downstream drug and immunofluorescence assays. This simple-to-follow protocol allows assessing drug sensitivity, on-target activity, and combined drug synergy.
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- Glioblastoma is the most common and lethal brain cancer, and despite progress in precision medicine, treatment options are still limited due to a lack of effective molecular testing.
- The study presents a new four-dimensional (4D) cell-culture array made with a special polymer that can transform shape to facilitate drug response testing in glioblastoma models.
- This innovative platform allows for quick evaluation of drug sensitivity and effectiveness, potentially guiding therapy choices based on genomic and drug testing, improving patient outcomes in glioblastoma treatment.