Separation of inorganic anions on a high capacity porous polymeric monolithic column and application to direct determination of anions in seawater.

A commercially available 4.6 mm id x 50 mm polymethacrylate-based monolithic strong anion exchange column (ProSwift SAX-1S) designed for the separation of proteins has been successfully used to separate small inorganic anions in the presence of a seawater sample matrix. Using a hydroxide eluent with suppressed conductivity detection the ion exchange capacity of this column declined over time; however, using KCl as the eluent, the column performance was stable with a capacity of 530 microequiv.

Polymerised bicontinuous microemulsions as stationary phases for capillary electrochromatography. Effect of pore size on chromatographic performance.

Monolithic columns for capillary electrochromatography (CEC) were prepared by in situ polymerisation of bicontinuous microemulsions containing butyl methacrylate. The resulting monoliths were found to be permeable to mobile phase flow and their behaviour as CEC stationary phases was investigated. It was found that the monoliths were able to separate a simple test mixture of phthalates, but that efficiencies were low.