Effects of age on optical coherence tomography measurements of healthy retinal nerve fiber layer, macula, and optic nerve head.

Purpose: To determine the effects of age on global and sectoral peripapillary retinal nerve fiber layer (RNFL), macular thicknesses, and optic nerve head (ONH) parameters in healthy subjects using optical coherence tomography (OCT).

Design: Retrospective, cross-sectional observational study.

Participants: A total of 226 eyes from 124 healthy subjects were included.

Repeated, noninvasive, high resolution spectral domain optical coherence tomography imaging of zebrafish embryos.

Purpose: To demonstrate a new imaging method for high resolution spectral domain optical coherence tomography (SD-OCT) for small animal developmental imaging.

Methods: Wildtype zebrafish that were 24, 48, 72, and 120 h post fertilization (hpf) and nok gene mutant (48 hpf) embryos were imaged in vivo. Three additional embryos were imaged twice, once at 72 hpf and again at 120 hpf.

Three-dimensional optical coherence tomography (3D-OCT) image enhancement with segmentation-free contour modeling C-mode.

Purpose: To develop a semiautomated method to visualize structures of interest (SoIs) along their contour within three-dimensional, spectral domain optical coherence tomography (3D SD-OCT) data, without the need for segmentation.

Methods: With the use of two SD-OCT devices, the authors obtained 3D SD-OCT data within 6 x 6 x 1.4-mm and 6 x 6 x 2-mm volumes, respectively, centered on the fovea in healthy eyes and in eyes with retinal pathology.

Validation of spectral domain optical coherence tomographic Doppler shifts using an in vitro flow model.

Purpose: To validate velocity measurements produced by spectral domain optical coherence tomography (SD-OCT) in an in vitro laminar flow model.

Methods: A 30-mL syringe filled with skim milk was inserted into a syringe pump. Intravenous (i.

Ultrahigh-resolution spectral domain optical coherence tomography imaging of the lamina cribrosa.

Study of the structure of the lamina cribrosa is critical in glaucoma research. The purpose of this study is to determine the optimal spectral domain optical coherence tomography imaging protocol for the digital isolation and display of the lamina cribrosa. Three-dimensional datasets centered on the lamina cribrosa were obtained with 200 X 200 to 512 X 512 A-scan densities.

Assessing the relationship between central corneal thickness and retinal nerve fiber layer thickness in healthy subjects.

Purpose: To determine the relationship between central corneal thickness (CCT) and retinal nerve fiber layer (RNFL) thickness obtained by scanning laser polarimetry (GDx-VCC; Carl Zeiss Meditec, Dublin, California, USA), confocal scanning laser ophthalmoscopy (HRT II; Heidelberg Engineering, Heidelberg, Germany), and optical coherence tomography (Stratus OCT; Carl Zeiss Meditec).

Design: Multicenter clinical trial, retrospective cross-sectional study.

Methods: One hundred and nine healthy subjects from the Advanced Imaging in Glaucoma Study were enrolled in this study.

Optical coherence tomography scan circle location and mean retinal nerve fiber layer measurement variability.

Purpose: To investigate the effect on optical coherence tomography (OCT) retinal nerve fiber layer (RNFL) thickness measurements of varying the standard 3.4-mm-diameter circle location.

Methods: The optic nerve head (ONH) region of 17 eyes of 17 healthy subjects was imaged with high-speed, ultrahigh-resolution OCT (hsUHR-OCT; 501 x 180 axial scans covering a 6 x 6-mm area; scan time, 3.

Clinical application of MRI in ophthalmology.

MRI has long been applied to clinical medical and neurological cases for the structural assessment of tissues as well as their physiological and functional needs and processes. These uses are at a variety of developmental stages in ophthalmology, from common use of clinical structural assessment for neuro-ophthalmology and evaluation of space-occupying lesions to the beginning stages of experimentally measuring functional activation of specific layers within the retina and measurement of physiological oxygen responses. New MRI methodologies, such as the use of orbital coils and Gd-DTPA image enhancement, have been researched, developed, and validated in the eye, opening new possibilities for this technology to enter the clinic.

Automated assessment of the optic nerve head on stereo disc photographs.

Purpose: To develop automated software for optic nerve head (ONH) quantitative assessment from stereoscopic disc photographs and to evaluate its performance in comparison with human expert assessment.

Methods: A fully automated system, including three-dimensional ONH modeling, disc margin detection, cup margin detection, and calculation of stereometric ONH parameters, was developed and tested. One eye each from 54 subjects (23 healthy, 17 suspected glaucoma, and 14 glaucoma) was enrolled.

Spectral oximetry assessed with high-speed ultra-high-resolution optical coherence tomography.

We use Fourier domain optical coherence tomography (OCT) data to assess retinal blood oxygen saturation. Three-dimensional disk-centered retinal tissue volumes were assessed in 17 normal healthy subjects. After removing DC and low-frequency a-scan components, an OCT fundus image was created by integrating total reflectance into a single reflectance value.