Publications by authors named "Kelly A Giffear"
Perinatal exposure of rats and mice to the typically reported 4mg/g bd wt dose of monosodium glutamate (MSG) results in a complete block in GH secretion as well as obesity, growth retardation and a profound suppression of several cytochrome P450s, including CYP2C11, the predominant male-specific isoform--all irreversible effects. In contrast, we have found that a lower dose of the food additive, 2mg/g bd wt on alternate days for the first 9days of life results in a transient neonatal depletion of plasma GH, a subsequent permanent overexpression of CYP2C11 as well as subnormal (mini) GH pulse amplitudes in an otherwise normal adult masculine episodic GH profile. The overexpressed CYP2C11 was characterized by a 250% increase in mRNA, but only a 40 to 50% increase in CYP2C11 protein and its catalytic activity.
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- Endocrine disruptors like 17β-estradiol (E2) and estriol (E3) are common environmental pollutants linked to health risks, with their interactions with dissolved organic matter (DOM) affecting their concentration and impact.
- Researchers investigated how humic acid (HA), a component of DOM, binds to E2 and E3 using nuclear magnetic resonance (NMR) spectroscopy, discovering that binding primarily occurs at the aromatic ring even at low HA concentrations.
- Their findings were supported by additional assays, enhancing understanding of estrogen-HA interactions and showcasing the effectiveness of combining biochemical and analytical methods to study these binding processes.