Isolation and short-term culturing of primary lymphatic endothelial cells from collecting lymphatics: A techniques study.

Objective: To develop an experimental method for routine isolation and short-term culture of primary lymphatic endothelial cells from specific collecting vessels.

Methods: Lymphatic endothelial cell tubes (LECTs) were isolated from micro-dissected collecting vessels. LECTs were allowed to attach and grow for ~3 weeks before being passaged.