Effect of gastric bypass and gastric banding on proneurotensin levels in morbidly obese patients.

Context: Neurotensin is produced mainly in the N cells of the ileum and has a role in appetite regulation; levels are decreased in obese subjects and increase after bariatric surgery. Mature neurotensin is very unstable, with a short half-life.

Objective: The objective of this study was to compare baseline and postoperative levels of the more stable neurotensin precursor, proneurotensin/neuromedin (pro-NT/NMN), in patients after gastric banding, gastric bypass, and nonoperated controls, respectively, during long-term follow-up.

Breakpoint locations within chromosomes 1, 2, and 4 of patients with increased radiosensitivity.

The exposure to low LET-radiation leads to a relative homogeneous distribution of initial damage at the DNA. Subsequent repair and post-repair mechanisms might lead to a selection of specific breakpoint locations along chromosomes. Cells from patients with increased radiosensitivity may have more specific breakpoints due to impaired repair mechanisms.

Effect of sibutramine and of cognitive-behavioural weight loss therapy in obesity and subclinical binge eating disorder.

Aim: In this randomized, double-blind, placebo-controlled study, the effect of sibutramine and cognitive-behavioural weight loss (cognitive-BWL) treatment was assessed in obese subjects with and without subclinical binge eating disorder (sBED).

Methods: Seventy-three obese participants were recruited from the community, 29 with and 44 without sBED. Subjects were randomly assigned to a 16-week treatment with either sibutramine or placebo while simultaneously participating in a cognitive-behavioural weight loss treatment.

Global mineralogical and aqueous mars history derived from OMEGA/Mars Express data.

Global mineralogical mapping of Mars by the Observatoire pour la Mineralogie, l'Eau, les Glaces et l'Activité (OMEGA) instrument on the European Space Agency's Mars Express spacecraft provides new information on Mars' geological and climatic history. Phyllosilicates formed by aqueous alteration very early in the planet's history (the "phyllocian" era) are found in the oldest terrains; sulfates were formed in a second era (the "theiikian" era) in an acidic environment. Beginning about 3.

Identification of the cytochrome P450 monooxygenase that bridges the clavine and ergoline alkaloid pathways.

Clavines and D-lysergic acid-derived alkaloid amides and alkaloid peptides are two different families of compounds that have the indole-derived tetracyclic metergoline ring system in common. Previous work has shown that D-lysergic acid is biosynthetically derived from clavine alkaloids. Recent cloning and analysis of the ergot alkaloid biosynthesis gene cluster from the D-lysergic acid peptide (ergopeptines)-producing Claviceps purpurea, has shown that it most probably contains all genes necessary for D-lysergic acid synthesis as well as those that encode the assembly of D-lysergic acid peptides, such as ergotamine.

Effects of low-carbohydrate vs low-fat diets on weight loss and cardiovascular risk factors: a meta-analysis of randomized controlled trials.

Background: Low-carbohydrate diets have become increasingly popular for weight loss. However, evidence from individual trials about benefits and risks of these diets to achieve weight loss and modify cardiovascular risk factors is preliminary.

Methods: We used the Cochrane Collaboration search strategy to identify trials comparing the effects of low-carbohydrate diets without restriction of energy intake vs low-fat diets in individuals with a body mass index (calculated as weight in kilograms divided by the square of height in meters) of at least 25.

Body composition and adiponectin serum concentrations in adult patients with cystic fibrosis.

Context: Cystic fibrosis (CF) is a disease characterized by weight loss and chronic low-grade inflammation.

Objective: The objective of the study was to assess changes in body composition and the serum concentrations of adiponectin, a marker of negative energy balance and insulin sensitivity, in adult patients with CF.

Design: This was a cross-sectional study.

Human adipose tissue-derived mesenchymal stem cells differentiate into insulin, somatostatin, and glucagon expressing cells.

Mesenchymal stem cells (MSC) from mouse bone marrow were shown to adopt a pancreatic endocrine phenotype in vitro and to reverse diabetes in an animal model. MSC from human bone marrow and adipose tissue represent very similar cell populations with comparable phenotypes. Adipose tissue is abundant and easily accessible and could thus also harbor cells with the potential to differentiate in insulin producing cells.

Menstrual cycle symptoms are associated with changes in low-grade inflammation.

Background: A close link between mood, low-grade inflammation and obesity has been demonstrated even in healthy subjects. We investigated the relationship between changes in physical and psychological symptoms and inflammatory markers during the menstrual cycle both in normal weight and in overweight women.

Materials And Methods: Eight healthy normal weight (body mass index 21.

Maternal HIV type 1 infection suppresses MMP-1 expression in endothelial cells of uninfected newborns: nonviral vertical transmission of HIV type 1-related effects.

HIV-1 infection is associated with vascular alterations. This is accompanied by an increased risk of cardiovascular diseases and Kaposi's sarcoma, an endothelial cell-derived tumor. We investigated the impact of maternal HIV-1 infection on phenotype and gene expression of endothelial cells in newborns.

Cytokine-induced metabolic effects in human adipocytes are independent of endogenous nitric oxide.

Nitric oxide (NO) has been recognized as a potential mediator of inflammation-induced metabolic alterations, including insulin resistance. However, expression mechanisms and potential roles of endothelial and inducible NO synthases (eNOS and iNOS, respectively) in human adipocytes are poorly understood. In the present study, we aimed to analyze several aspects of NO-related gene expression and metabolite synthesis in basal and inflammation-activated human adipocyte models.

Phyllosilicates on Mars and implications for early martian climate.

The recent identification of large deposits of sulphates by remote sensing and in situ observations has been considered evidence of the past presence of liquid water on Mars. Here we report the unambiguous detection of diverse phyllosilicates, a family of aqueous alteration products, on the basis of observations by the OMEGA imaging spectrometer on board the Mars Express spacecraft. These minerals are mainly associated with Noachian outcrops, which is consistent with an early active hydrological system, sustaining the long-term contact of igneous minerals with liquid water.

Measurement of the spectrally-resolved absolute phase difference between orthogonal optical modes using a nonlinear beat signal.

On the basis of white-light interferometry with spectrally integrated detection and Fourier transform (FT) analysis, we demonstrate a novel technique for measuring the spectrally-resolved absolute phase difference between orthogonal optical modes with milliradian precision. The phase difference is evaluated from a nonlinear beat signal, occurring in the phase spectrum when independent interferograms, formed by individual modes, are recorded simultaneously. Although scanning white-light FT interferometry is a linear technique in general, the nonlinear beat signal is due to spectral amplitude variations in each mode.

Phosphinothricin tripeptide synthetases in Streptomyces viridochromogenes Tü494.

The tripeptide backbone of phosphinothricin (PT) tripeptide (PTT), a compound with herbicidal activity from Streptomyces viridochromogenes, is assembled by three stand-alone peptide synthetase modules. The enzyme PhsA (66 kDa) recruits the PT-precursor N-acetyl-demethylphosphinothricin (N-Ac-DMPT), whereas the two alanine residues of PTT are assembled by the enzymes PhsB and PhsC (129 and 119 kDa, respectively). During or after assembly, the N-Ac-DMPT residue in the peptide is converted to PT by methylation and deacetylation.

Randomized controlled clinical trial of Blood Glucose Awareness Training (BGAT III) in Switzerland and Germany.

Although both diabetes and the efficacy of medical management are international issues, psycho-educational interventions might be culturally bound. Blood Glucose Awareness Training (BGAT) is a psycho-educational program for patients with type 1 diabetes mellitus. It is focused on improving recognition and management of extreme blood glucose levels, and is the best documented American psycho-educational program for this purpose.

X-PERT: weight reduction with orlistat in obese subjects receiving a mildly or moderately reduced-energy diet: early response to treatment predicts weight maintenance.

Aim: To determine the effect of two different levels of energy deficit on weight loss in obese patients treated with orlistat.

Methods: Patients (n=430) were randomized in a 1-year, multicentre, open-label, parallel group study conducted at 23 hospital centres and university medical departments worldwide. Obese outpatients (body mass index 30--43 kg/m(2)) aged 18--70 years with a body weight of >or=90 kg and a waist circumference of >or=88 cm (women) or >or=102 cm (men) were treated with orlistat 120 mg three times daily plus a diet that provided an energy deficit of either 500 or 1,000 kcal/day for 1 year.

[Low-carbohydrate diets for obesity].

Low-carbohydrate diets for the treatment of obesity are currently receiving widespread popularity, mainly in Anglo-Saxan countries. Several controlled trials have demonstrated larger weight loss (3-6 kg) during these diets compared to conventional low-fat diets. The weight differences between these diets were statistically significant after 6 months but not any more after 12 months.

The European view of hospital undernutrition.

Disease-related undernutrition is significant in European hospitals but is seldom treated or prevented. In 1999, the Council of Europe decided to collect information regarding nutrition programs in hospitals, and for this purpose, a network consisting of national experts from 12 of the Partial Agreement member states was established. The aim was to review the current practices in Europe regarding hospital food provision, to highlight deficiencies, and to issue recommendations to improve the nutritional care and support of hospitalized patients.

Pulse self-compression to the single-cycle limit by filamentation in a gas with a pressure gradient.

We calculate pulse self-compression of a 30 fs laser pulse traversing gas with different pressure gradients. We show that an appropriate density profile brings significant improvement to the self-compression by filamentation. Under an optimal pressure gradient, the pulse duration is reduced to the single optical cycle limit over a long distance, allowing easy extraction into an interaction chamber.

Generation of intense few-cycle laser pulses through filamentation - parameter dependence.

Intense few-cycle laser pulses as short as 5.1 fs are generated though self-filamentation in a noble gas atmosphere. We study the dependence of the laser pulse fidelity on the driving pulse profile and chirp as well as on the gas parameters, quantify their pointing stability and spatial quality.

What are disease perceptions and subjective treatment goals of insulin treated diabetic patients?

Background: Despite increasing importance of patient self-management, little is known about their own perceptions and treatment goals.

Objectives: The aim of this explorative study was to examine what diabetic patients perceive as most concerning and what their own treatment goals are.

Methods: A 23-item anonymous questionnaire was distributed among type 1 diabetic patients treated with and without an insulin pump and insulin treated type 2 diabetic patients in the outpatient clinic of a University Hospital.

Central fat excess in polycystic ovary syndrome: relation to low-grade inflammation and insulin resistance.

Background: It is controversial whether the polycystic ovary syndrome (PCOS) per se increases low-grade chronic inflammation and whether this relates to central fat excess. In addition, the association between circulating sex hormones and body fat distribution in premenopausal women is debated.

Methods: Blood was drawn from 20 patients with PCOS and compared with 15 controls, matched for body mass index and age.

Characterization of ultrashort optical pulse properties by amplitude-modulation-balanced heterodyne gating.

We present a general approach for the measurement of the properties of optical pulses by exploiting features of the optical carrier domain. We demonstrate the principle of a novel balanced detection scheme that avoids the difficulties associated with homodyne detection in the baseband used in linear optical sampling methods so far. The residual timing instability of the repetition rate synchronization between mode-locked lasers is measured with the new detection technique.

Frequency-sheared, time-delayed extreme-ultraviolet pulses produced by high-harmonic generation in argon.

We report the production of frequency-sheared high harmonics in argon by control of the envelope and chirp of the electric field of the femtosecond driving laser pulse. Using the classic three-step model of high-harmonic generation, we established a direct link between the properties of the harmonics and the fully characterized driving pulses. A simulation of the single-atom response in the strong-field approximation confirms the simple picture and shows good agreement with the experimental results.

The transcriptional repressor Nab1 is a specific regulator of pathological cardiac hypertrophy.

Hypertrophy represents the major physiological response of the heart to adapt to chronically enhanced workload, but is also crucial in the development of heart failure. Although we know of numerous inducers of cardiac hypertrophy, little is known about mechanisms that limit cardiac hypertrophy. Here, we describe the transcriptional repressor NAB1 as an endogenous regulator of cardiac growth.