Rational design of an ultrabright quinolinium-fused rhodamine turn-on fluorescent probe for highly sensitive detection of SO derivatives: Applications in food safety and bioimaging.

Sulfur dioxide (SO) is an essential signaling molecule involved in various physiological processes within living organisms. Bisulfite (HSO) possesses antioxidant, antimicrobial, and preservative properties, making it a common food additive. However, elevated levels of SO or excessive HSO intake can lead to a range of diseases, highlighting the importance of detecting SO and its derivatives (HSO/SO).

Ultrafast detection of bisulfite by a unique quinolinium-based fluorescent probe and its applications in smartphone-assisted food detection and bioimaging.

Sulfur dioxide (SO) is one of the major pollutants in the atmosphere, which is highly susceptible to inhalation by the human body and is converted into its derivatives (HSO/SO), which is hazardous to both human health and the ecological environment. Therefore the detection of SO derivatives (HSO/SO) is very important. In this work, we have prepared ID-QL, a water-soluble fluorescent probe based on the intramolecular charge transfer (ICT) mechanism, it exhibits colorimetric and fluorescent dual-channel response to HSO with ultrafast, highly selective and sensitive detection.

Fluorescent probe for imaging NH in plants, food, and living cells and for quantitative detection of NH in soil and water using a smartphone.

Hydrazine is volatile and highly toxic, causing severe harm to water, soil, air, and organisms. Therefore, real-time detection and long-term monitoring of hydrazine are crucial for environmental protection and human health. Herein, an "OFF-ON" fluorescent probe 5-((10-ethyl-2-methoxy-10 H-phenothiazin-3-yl)methylene)-2,2-dimethyl-1,3-dioxane-4,6-dione (MPD) for hydrazine detection through a nucleophilic addition reaction was developed.

A self-assembled nanoprobe for rapid detection of hypochlorite in pure water and its application in living cells, food and environmental systems.

As an important ROS species participating in various physiological and pathological processes, high level of hypochlorite (ClO) poses significant health and safety concerns, necessitating efficient detection methods. Herein, this study introduces a water-soluble fluorescent nanoprobe Nano-SJD, effectively detect ClO in both food samples and living cells. The small molecular probe SJD with N, N-dimethylthiocarbamyl (DMTC) as recognition moiety was constructed based on a naphthalene derivative.

A smartphone-adaptable fluorescent probe for visual monitoring of fish freshness and its application in fluorescent dyes.

Due to increasing food safety issues, developing intelligent, on-site, and visual methods for detecting fish freshness has attracted significant attention. Here, we have prepared a benzo[h]chromene derivative BCN that can visually detect 12 biogenic amines (BAs) with high sensitivity. The mechanism for recognizing cadaverine (Cad) is that the probe reacts with Cad to produce a Schiff base derivative, which alters the charge distribution within the molecule, resulting in significant colorimetric and fluorescence changes.

A sensing label or gel loaded with an NIR emission fluorescence probe for ultra-fast detection of volatile amine and fish freshness.

A simple benzopyran-based fluorescence probe DCA-Apa detection of volatile amine has been synthesized. DCA-Apa can recognize volatile amines by dual channel mode (changing from blue to light yellow in sunlight, and from weak pink to orange under 365 nm) in pure water system. DCA-Apa has the advantages of ultra-fast response (∼6 s), NIR emission (655 nm), and a good fluorescence response for many amines.

Visualizing the crucial roles of plasma membrane and peroxynitrite during abdominal aortic aneurysm using two-photon fluorescence imaging.

Peroxynitrite (ONOO) and cell plasma membrane (CPM) are two key factors in cell pyroptosis during the progression of abdominal aortic aneurysm (AAA). However, their combined temporal and spatial roles in initiating AAA pathogenesis remain unclear. Herein, we developed a two-photon fluorescence probe, BH-Vis, enabling real-time dynamic detection of CPM and ONOO changes, and revealing their interplay in AAA.

Clinical observation of extraction-site incisional hernia after laparoscopic colorectal surgery.

Background: Laparoscopic colorectal cancer surgery increases the risk of incisional hernia (IH) at the tumor extraction site.

Aim: To investigate the incidence of IH at extraction sites following laparoscopic colorectal cancer surgery and identify the risk factors for IH incidence.

Methods: This study retrospectively analyzed the data of 1614 patients who underwent laparoscopic radical colorectal cancer surgery with tumor extraction through the abdominal wall at our center between January 2017 and December 2022.

A novel fluorescence probe for ultrafast detection of SO derivatives/biogenic amines and its multi-application: Detecting food and fish freshness, fluorescent dye and bioimaging.

In this study, we have effectively prepared a novel fluorescent probe named HDXM based on benzopyran derivatives for the ultrafast detection (within 3 s) of SO derivatives or biogenic amines. HDXM showed a noticeable color change after the addition of SO derivatives (from purple to colorless) or biogenic amines (from purple to blue), indicating that HDXM can identify two analytes with the naked eye. It is worth noting that HDXM can be used to detect SO derivatives in actual sugar samples, and to image HSO/SO in living cells.

A colorimetric and NIR fluorescent probe for ultrafast detecting bisulfite and organic amines and its applications in food, imaging, and monitoring fish freshness.

Herein, for the first time, we have successfully constructed a novel near-infrared (NIR) emission fluorescent probe Dpyt for ultrafast detecting (within 5 s) bisulfate and organic amines based on a 1,2-dihydrocyclopenta[b]chromene-barbiturate conjugation system. Upon addition of bisulfate or organic amines, Dpyt displayed a distinct color change from blue to colorless or from purple to blue, respectively, suggesting that the Dpyt can be used to detect two analytes by the naked eye. Based on quantum chemistry calculations, the fluorescence quenching of Dpyt after the addition of HSO is caused by the photoinduced electron transfer (PET) process of the adduct Dpyt-HSO.

Clinical observation of laparoscopic seromuscular dissection in the treatment of gastric stromal tumors.

Objective: To investigate the safety and oncological prognosis of laparoscopic seromuscular dissection (LSD) in the treatment of gastric stromal tumors.

Material And Methods: From June 2016 to July 2022, 67 patients with gastric stromal tumors underwent laparoscopic seromuscular dissection (LSD), and 58 patients underwent non-LSD surgery during the same period (52 patients underwent laparoscopic gastric wedge resection (LWR), two patients underwent proximal gastrectomy, three patients underwent total gastrectomy, one patient underwent distal gastrectomy and partial hepatectomy). Gastric stromal tumor patients were compared to compare the surgical results, tumor relapse rate, and survival rate of the two groups.

A red fluorescence probe for reversible detection of HSO/HO and its application in food samples and bioimaging.

Reducing agent SO and oxidant HO are two essential substances in cells, and the balance between them is closely related to the survival of cells. SO derivative HSO is often used as food additive. Therefore, simultaneous detection of SO and HO is of great significance in biology and food safety.

An AIE mechanism-based fluorescent probe for relay recognition of HSO/HO and its application in food detection and bioimaging.

In view of the important physiological role of HSO and HO, it is of great significance to develop fluorescent probes to detect HSO and HO in aqueous medium. We herein report a new fluorescent probe (E)-3-(2-(4-(1,2,2-triphenylvinyl)styryl)benzo [d]thiazol-3-ium-3-yl)propane-1-sulfonate (TPE-y) possessing benzothiazolium salt based on tetraphenylethene (TPE) moiety with aggregation-induced emission (AIE) characteristics. TPE-y can sequentially recognize HSO and HO through colorimetric and fluorescence dual-channel response in HEPES (pH = 7.

Reversible colorimetric and NIR fluorescent probe for sensing SO/HO in living cells and food samples.

Preservative sulfur dioxide (SO) and bleach hydrogen peroxide (HO) were widely used in the food industry, at the same time, they were also a redox pair in biological systems. Therefore, the reversible sensing SO/HO was of great significance in food safety and biology. In this paper, a colorimetric and NIR fluorescent dual channels response probe (DCA-Bba) for SO/HO based on chromene-barbiturate was developed.

KIF4 enforces the progression of colorectal cancer by inhibiting the autophagy via activating the Hedgehog signaling pathway.

This work firstly scrutinized the effect of KIF4 on the progression of CRC. KIF4 expression in CRC clinical tissues and cells was evaluated by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blot. Cell counting kit-8 assay, Transwell invasion and migration assay were implemented to research the function of KIF4 on the proliferation, invasion and migration of CRC cells.

Novel Colorimetric and NIR Fluorescent Probe for Bisulfite/Sulfite Detection in Food and Water Samples and Living Cells Based on the PET Mechanism.

Despite their status of being widely used as food additives, bisulfite (HSO)/sulfite (SO) can pose serious health risks when they are excessively added. Therefore, it is vital to develop a new method for detecting HSO/SO in foodstuff. In this paper, a benzopyran-benzothiazole derivative (probe -) with near-infrared emission was designed and synthesized by constructing a "push-pull" electronic system.

On-package ratiometric fluorescent sensing label based on AIE polymers for real-time and visual detection of fish freshness.

Freshness is an important parameter of fish quality. This study aims to develop a ratiometric fluorescent sensing label that is responsive to volatile amines, affording real-time and visual detection of fish freshness. For developing of the sensing label, an aggregation-induced emissive (AIE) polymer was prepared from the stimuli-responsive polymer polymethacrylic acid and the AIE molecule tetraphenylethylene and coated on to filter paper with rhodamine B as an internal reference.

Mitochondria-Targeted Fluorescent Turn-On Probe for Rapid Detection of Bisulfite/Sulfite in Water and Food Samples.

Bisulfite (HSO)/Sulfite (SO) is widely used as a food additive, but excessive use often leads to serious consequences, so the detection of HSO/SO is of great importance. In this paper, a novel 1,4-diethylpiperazine-modified coumarin-benzopyran derivative (probe ) has been synthesized and characterized. In PBS (10 mM, pH = 7.

Expression Patterns of Glycosylation Regulators Define Tumor Microenvironment and Immunotherapy in Gastric Cancer.

Glycosylation (Glyc) is prevalently related to gastric cancer (GC) pathophysiology. However, studies on the relationship between glycosylation regulators and tumor microenvironment (TME) and immunotherapy of GC remain scarce. We extracted expression data of 1,956 patients with GC from eight cohorts and systematically characterized the glycosylation patterns of six marker genes into phenotype clusters using the unsupervised clustering method.

A fluorescent and colorimetric dual-recognition probe based on copper(II)-decorated carbon dots for detection of phosphate.

In the present article, we report a novel fluorescent and colorimetric dual-signal sensing probe based on a CD-Cu complex for the detection of the phosphate ion (Pi). The yellow fluorescent carbon quantum dots (CDs) were simply synthesized one-step hydrothermal treatment of -phenylenediamine (OPD) and 4-aminobutyric acid (GABA). The method was based on the combination of the CDs and Cu to form a coordination complex.

A Novel HO Generator for Tumor Chemotherapy-Enhanced CO Gas Therapy.

Carbon monoxide (CO) gas therapy is a promising cancer treatment. However, gas delivery to the tumor site remains problematic. Proper tunable control of CO release in tumors is crucial to increasing the efficiency of CO treatment and reducing the risk of CO poisoning.

A highly sensitive ratiometric fluorescent sensor for copper ions and cadmium ions in scallops based on nitrogen doped graphene quantum dots cooperating with gold nanoclusters.

Based on the electrostatic interaction, we constructed a ratiometric fluorescence nanomixture of graphene quantum dots-gold nanoclusters (GQDs-AuNCs) for the quantitative detection of Cu and Cd. When Cu or Cd was added into the reaction system, the fluorescence of GSH-AuNCs at 565 nm can be quenched by Cu and enhanced by Cd while the intensity of N-GQDs at 403 nm stayed constant. Under the optimized conditions, the fluorescence intensity ratio (I/I) of the GQDs-AuNCs system was proportional to the concentration of Cu and Cd in the range of 8×10 mol/L-6×10 mol/L and 1×10 mol/L-4×10 mol/L, respectively, with detection limits of 4.

Mitochondria-Targeted Red-Emission Fluorescent Probe for Ultrafast Detection of HS in Food and Its Bioimaging Application.

Hydrogen sulfide (HS) contributes to human health and prolongs the storage time of postharvest fruits and vegetables. At the same time, HS can cause a negative impact on some foodstuffs and beverages, so an efficient probe to detect HS is needed. Herein, a fluorescent turn-on responding probe for HS detection has been designed and synthesized.

Investigating phosphorescence capability of halogen-substituted metal-free organic molecules: A theoretical study.

The radiative and non-radiative decay processes of five compounds are investigated through a comprehensive computational approach, for the aim of investigating the effect of different halogen substituents to the phosphorescent emission. Their optimal configurations at the ground (S) and lowest triplet excited (T) states are obtained and the calculated phosphorescent emission spectra are comparable with the experimental values. For 1,4-di(9H-carbazol-9-yl)benzene (PDCz), the electronic transition is between the highest occupied molecular orbital (HOMO) and the lowest unoccupied molecular orbital (LUMO), while for the four halides, the electronic transitions are attributed to several molecular orbitals.