Publications by authors named "Keli L Rodriguez"
In nucleosomes, histone N-terminal tails exist in dynamic equilibrium between free/accessible and collapsed/DNA-bound states. The latter state is expected to impact histone N-termini availability to the epigenetic machinery. Notably, H3 tail acetylation (e.
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- - Acetylation of lysine 16 on histone H4 (H4K16ac) is influenced by the histone acetyltransferase KAT8, which plays different roles depending on the protein complex it's part of (NSL or MSL).
- - KAT8, when associated with the NSL complex, adds acetyl groups to H4K5 and H4K8, which are important for activating genes necessary for cell survival.
- - Interestingly, while H4K16ac and MSL complex proteins don't affect cell growth or chromatin structure, the NSL complex is crucial for stimulating transcription of housekeeping genes.
Chromatin immunoprecipitation (ChIP) is a method used to examine the genomic localization of a target of interest (e.g., proteins, protein posttranslational modifications, or DNA elements).
View Article and Find Full Text PDFBackground: Plant homeodomain (PHD) fingers are central "readers" of histone post-translational modifications (PTMs) with > 100 PHD finger-containing proteins encoded by the human genome. Many of the PHDs studied to date bind to unmodified or methylated states of histone H3 lysine 4 (H3K4). Additionally, many of these domains, and the proteins they are contained in, have crucial roles in the regulation of gene expression and cancer development.
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