Publications by authors named "Keiti O Alessio"

Rapid industrialization results in the production of large quantities of waste that are commonly discharged into water bodies, leading to the damage of the aquatic ecosystem and freshwater organisms. Copper (Cu) can induce oxidative damage in fish muscle, the main fish portion that is consumed by humans. However, the responses of the Amazon fish Cichlasoma amazonarum and its capacity to withstand acute Cu concentrations found in Amazon water around mines remain unknown.

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Article Synopsis
  • The study investigates how environmentally relevant copper (Cu) concentrations affect the metabolism of the fish Cichlasoma amazonarum, focusing on energy production and depletion of ATP.
  • Exposure to 750 and 1500 μg/L Cu resulted in significantly reduced activity of key enzymes, such as branchial mitochondrial creatine kinase and pyruvate kinase, alongside lower ATP levels, indicating metabolic dysfunction.
  • Increased levels of reactive oxygen species and lipid peroxidation were observed in fish exposed to Cu, suggesting that oxidative stress plays a role in bioenergetic imbalance and impairment in energy homeostasis.
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This work proposes a simple device for thermometric titration using a contactless sensor. A low-cost infrared sensor (around $5) was adapted in a disposable, polystyrene vessel for monitoring the temperature to determine the endpoint of neutralization, redox, and complexation titrations, using a homemade syringe pump to titration control. Open-source software was developed to control the device using the Raspberry Pi platform and to perform automatic endpoint determination.

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A simple, rapid, low-cost method was proposed for the imaging of biofilms on metallic surfaces using an infrared camera. Stainless steel coupons were cooled to generate a thermal gradient in relation to biofilm for active thermography (AT). Both cooling and image acquisition times were optimized and the images obtained with AT were compared with those from scanning electron microscopy.

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This work developed a new technique and an application of an existing approach to determine sodium in food sauces, involving enthalpimetric reactions in the infrared. Infrared Thermometric Titration (TT-IR) was utilized, with simple analyzers and low-cost measurement instruments for the acquisition of the surface temperature generated in the sodium precipitation reaction and development of software for the acquisition and processing of data using Raspberry Pi. The sodium was also quantified by Thermal Infrared Enthalpimetry (TIE), a recently developed technique.

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Paper microzone plates and thermal infrared enthalpimetry (TIE) were combined with potato juice as natural reagent extract to perform the determination of hydrogen peroxide in pharmaceutical, bleaching, and toiletry products. A multichannel pipette was used for reagent addition simultaneously in multiple zones of paper devices, and the temperature rise was determined using an infrared camera. In order to provide suitable measurements, some parameters were optimized such as pH, volume of reagents, and stability of the extract.

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