Publications by authors named "Keitaro Fukabori"
8-oxo-2'-deoxyguanosine (8-oxo-dG) is a nucleoside resulting from oxidative damage and is known to be mutagenic. 8-Oxo-dG has been related to aging and diseases, including neurological disorders and cancer. Recently, we reported that a fluorescent nucleoside derivative, adenosine-1,3-diazaphenoxazine (Adap), forms a stable base pair with 8-oxo-dG in DNA with accompanying efficient quenching.
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- Researchers have developed a new fluorescence resonance energy transfer (FRET) probe using Adap, which is an artificial nucleoside that can specifically bond through multiple hydrogen bonds and is selectively quenched by 8-oxo-2'-deoxyguanosine (8-oxo-dG) in DNA.
- The probe consists of two strands: one with Adap and the other with natural nucleotides, designed to create a less stable double strand that initially shows quenching of fluorescence due to FRET.
- When 8-oxo-dG is present in the complementary DNA strand, it enhances the separation of the duplex probe, leading to fluorescence recovery and demonstrating the probe’s ability to distinguish between 8-oxo-dG and regular de